Characterization of Nanomaterials in Liquid Phase for Nanotoxicological Assessment

Abstract

Nanosized particles are currently of interest due to their attractive physical, photochemical, and catalytic properties. However, such small size particles could possess not only unique and advantageous properties, but could also have potentially new types of toxicity to humans. To estimate the true nano toxicity, it is therefore improtant to perform carful characterization of nano colloidal suspension. To elucidate important parameters for nano toxicity assessment of nano colloidal materials, experiments were carried out using pulsed field gradient nuclear magnetic resonance (PFG-NMR), asymmetrical flow field-flow fractionation (AFFFF), and dynamic light scattering (DLS) methods. For the assessment of the in nano toxicity, the amounts of total and bulk bovine serum albumin (BSA) molecules in nano colloidal suspensions were determined using the PFG-NMR and AFFFF methods. Because the amount of bulk BSA molecules in the cell culture medium is a significant factor in inducing cell growth and because BSA can strongly adsorb onto the nano sized particles, this value is an important parameter for in vitro toxicological assessment. In addition, structural analysis of the colloidal nanomaterials were successfully performed using AFFFF-multi angle light scattering (MALS) and DLS methods. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/35139