Use of the Transmission Electron Microscopy for Examination of Biofilms Structure

Asaulenko, L.G.; Purish, L.M.; Abdulina, D.R.

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Title	Use of the Transmission Electron Microscopy for Examination of Biofilms Structure
Authors	Asaulenko, L.G. Purish, L.M. Abdulina, D.R.
ORCID
Keywords	Transmission electron microscopy (TEM) Biofilm Lectins EPS
Type	Conference Papers
Date of Issue	2013
URI	http://essuir.sumdu.edu.ua/handle/123456789/35267
Publisher	Sumy State University
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Citation	Asaulenko, L.G. Use of the Transmission Electron Microscopy for Examination of Biofilms Structure [Текст] / L.G. Asaulenko, L.M. Purish, D.R. Abdulina // Nanomaterials: Applications & Properties (NAP-2013) : 2-nd International conference, Alushta, the Crimea, September 17-22, 2012 / Edited by: A. Pogrebnjak. - Sumy : Sumy State University, 2013. - V.2, No1. - 01PCSI10
Abstract	Combination of transmission electron microscopy technics with colloidal gold labeled lectin staining allowed investigating glycoconjugates in biofilms of the corrosion relevant bacteria. Lectins with the same specificity were found to express different affinity to the compounds of the bacterial biofilms. LBA lectin showed the highest binding capacity to N-acetyl-D-galactosamine in Desulfovibrio sp. 10 and Bacillus subtilis 36 biofilms. For the assessment of N-acetyl-D-glucosamine presence in Bacillus subtilis 36 and Pseudomonas aeruginosa 27 biofilms WGA lectin was the most efficient. Staining with lectins labeled with the colloidal gold was proved to be a promising express technique for the investigations of glycoconjugate distribution and localization in bacterial biofilms. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/35267
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