The Method for Protein Display on the Surface of Bacterial Magnetic Nanoparticles

Grouzdev, D.S.; Dziuba, M.V.; Kurek, D.V.; Ovchinnikov, A.I.

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Title	The Method for Protein Display on the Surface of Bacterial Magnetic Nanoparticles
Authors	Grouzdev, D.S. Dziuba, M.V. Kurek, D.V. Ovchinnikov, A.I.
ORCID
Keywords	Magnetosomes Antibody immobilization Protein display Mistic AFM
Type	Conference Papers
Date of Issue	2013
URI	http://essuir.sumdu.edu.ua/handle/123456789/35488
Publisher	Sumy State University
License	Copyright not evaluated
Citation	The Method for Protein Display on the Surface of Bacterial Magnetic Nanoparticles [Текст] / D.S. Grouzdev, M.V. Dziuba, D.V. Kurek, A.I. Ovchinnikon // Nanomaterials: Applications & Properties (NAP-2013) : 3-nd International conference, Alushta, the Crimea, September 16-21, 2013 / Edited by: A. Pogrebnjak. - Sumy : Sumy State University, 2013. - V.2, No4. - 04NABM27
Abstract	In this study, we present a comprehensive approach to the design and development of magnetosomes with antibodies immobilized on their surface by integration in membrane in vitro. Designed fusion proteins Mbb and Mistbb consisted of anchor proteins and BB-domains of Staphylococcus aureus protein A as IgG-binding region were used for development of IgG-binding magnetosomes. The magnetosome membrane protein MamC and membrane protein of Bacillus subtilis Mistic were selected as anchor proteins. Using Response Surface Methodology (RSM), the high level of fusion proteins integration into bacterial nanopar-ticles membrane was achieved. IgG-binding magnetosomes obtained through this strategy could serve as multifunctional platform for displaying various types of antibodies. Such systems could be applied as theranostic agents. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/35488
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