Identification of epulis patients with a high risk of periodontal diseases: DNA infrared spectra, expression MGMT and p53 by and apoptosis analysis

Abstract

Background The objective of this study was to epulis apoptosis analyze, expression of MGMT and p53, DNA methylation patients with a high risk of developing bone resorption and inflammatory periodontal diseases. Methods. Human epulis benign were assessed for their ability to expression of MGMT, p53. 51 epulis DNA were compared by infrared spectroscopy for their ability to apoptosis induced. We conducted a comparative analysis of global expression changes in human epulis using K-S test and Student method . Results. MGMT and p53 was expressed in giant-cell. Expression of MGMT and p53 in giant-cell epulis. By immunohistochemistry, 97.1±0.42% (P<0.001) of giant-cells were positive for MGMT, whereas only 6.21±0.26% of giant-cells were positive for p53 (P<0.001). Induction of the enzymatic activity of MGMT was increased p53. This could be explained by the fact that MGMT is physiologically expressed by the giant-cell of epulis, p53 expression is weak or absent in giant-cell epulis and non induced apoptosis in the fibrous connective tissue. Significant changes were observed IR absorption bands at - δsSN3 group bases. In intact periodontium in CH3 - IR bands were unchanged. Center band oscillations - δsSN3 group was at 1375 ± 1cm - 1. Percentage intensity on a scale transmission of infrared radiation was 7,18 ± 0,74%. Percentage of infrared absorption bands 1375 ± 1 cm- 1 in giant cell epulis equal to 13,24±3,7% (** P = 0.01). Changes in IR absorption bands observed in δsCH2 group. In intact periodontal δsCH2 next strip - strip center fluctuations - 1464 cm- 1, the percentage of intensity on a scale transmission of infrared radiation - 0,24 ± 0,03%. Percentage of IR absorption bands - 1464 cm-1 in the giant cell epulis was 0,46±0,09% (* P = 0.05). Percent transmittance intensity on a scale stretching, deformation and rocking vibrations of CH3 and CH2 groups of DNA Conclusion. Aim of this study was to find if IR can be used in neoplasm process DNA methylation detection. Statistical analysis shows that there are differences between spectra of giant cell epulis and control group. Fitting analysis allows to follow small changes in spectra CH3 groups. Presented results prove that infrared spectroscopy could be useful tool in DNA methylation. Morphologically, apoptosis is characterized by DNA fragmentation and formation of apoptotic. DNA CH3 groups can be detected by infrared spectra leads of breaks in DNA strands. This leads to the activation of apoptosis via p53 and MGMT.