Морфологические особенности белой пульпы селезенки новорожденных крысят после введения ксеногенной цереброспинальной жидкости

Abstract

У статті наведені морфологічні особливості білої пульпи селезінки (а саме лімфатичних вузликів) новонароджених щурів в нормі, а також після трьох- та десятикратного введення ксеногенної цереброспінальної рідини. Доведено, що парентеральне введення КЦСР новонародженим щурам сприяє ільш швидкому розвитку білої пульпи селезінки та збільшенню її відносної площі.

В работе представлены морфометрические особенности белой пульпы (в частности лимфатических узелков) селезенки новорожденных крысят в норме, а так же после трех- и десятикратного введения ксеногенной цереброспинальной жидкости. Доказано, что парэнтеральное введение КЦСЖ новорожденным крыятам способствует более быстрому развитию белой пульпы селезенки и увеличению ее относительной площади.

The spleen is the largest organ of the peripheral immune system in the human body that makes the significant contribution to the immune response, the quantitative and qualitative composition of the immune cells of blood, lymph and other lymphoid organs [3-5]. The influence of various chemical and biological substances on spleen was investigated [2]. Of particular interest among them is xenogenic cerebrospinal fluid (XCSF). The influence of XCSF on the morpho-functional features of ovaries, adrenal glands, bone marrow, mesenteric lymph nodes [1] were revealed, but the structural changes of the spleen after injections of XCSF was not studied. In the early postnatal period there is a rapid development of the lymphoid organs, including spleen, and therefore the response to various factors is expressed. Therefore, the aim of our work was to investigate the histomorphological features of neonatal rat spleen after three and ten times injection of xenogenic cerebrospinal fluid. The experiment was conducted on Wistar rats of both sexes weighing 10 to 45 g, depending on the time of sampling of the material. All exposures were carried out in accordance with "The international guidelines for biomedical research using laboratory animals." All animals were divided into 2 groups: control and experimental. Each group had two subgroups of six animals, the first of which was administered parenterally saline or XCSF three times, the second one 10 times exposure, every third day, in a dose of 2 ml/kg of body weight. For histological examinations thin slices 3-5 mm thick were made, followed by staining with hematoxylin-eosin. Based on the data the conclusion was made that triple parenteral administration of XCSF for newborn rats promoted more rapid development of the white pulp of the spleen. Its relative area increased by 60%. By the thirtieth day of life the majority of nodules of white pulp of the spleen already had separate zones. There were secondary nodules containing lymphocytes productive centers. Repeated administration of XCSF for newborn rats caused an increase in the relative area of the white pulp by 18%, while the average square of lymph nodules increased by 44.89% compared to the control group.