Immunohistochemical study of p53 and Ki-67 expression in gingival

Abstract

Objectives. The objective of this study was to analyze the expression of Ki-67 and p53 in gingival epithelium. Material and Methods. 29 pieces of periodontal tissues of patients who had died in Sumy Regional Hospital were evaluated for revealing Ki-67 and p53 by immunohistochemistry. Results. Patients who had died from complications of atherosclerosis by immunohistochemistry basal cells layer were positive 93.2±1.9% (P<0.05) for Ki-67, whereas only 50.72±0.5% of their gingival were positive for p53 (P<0.05) With respect to the immunoexpression of Ki-67 in gingival of not atherosclerotic patients they had 39.85±2.77% (P<0.01) of positive cells. In the gingival of not atherosclerotic patients presence of p53 was positive 2.07±0.6% (P<0.05) Conclusions. Proliferative gene Ki-67 expression among patients with not atherosclerotic conditions was low. Patients, who had died from complications of atherosclerosis with cellular hyperplasia, since it is merely an adaptive process, indicated an increased Ki-67 Gene and p53 expression. Among patients with atherosclerosis it is linked to cell’s arrests in the G1 phase of the cell cycle and that provides time for repair of the damaged DNA before entry into S or induction of apoptosis.