Hsp 90 overexpression in chronic bacterial prostatitis with corpora amylacea

Abstract

Objective: To study the expression of heat shock protein Hsp90 in patients with chronic bacterial prostatitis (CBP) and corpora amylacea formation. Method: Hsp90 expression was investigated in tissue of prostate of 22 CBPs with corpora amylacea by immunohistochemistry. Samples were fixed, embedded in paraffin and analized for Hsp90 accumulation using the anti-Hsp90 antibody, followed by DAB detection substrate and coun­terstained with Mayer’s hematoxylin. Microbiological examinations were carried out in intraoperative collection o f material. The identification of accumulated bacterial cultures was carried out using conventional methods based, on morphological, tinctirial, cultural, biochemical and antigenic properties. Results: In prostates with CBP E.coli was defined in 63.6 % of cases, S.aureus and P. vulgaris in 9.1 % of patients, Klebsiella spp. in 18.2 % of samples. CBP was characterized by significant inflammatory infiltration around glands and in the stroma. Immunohistochemical examination re­vealed significant expression of Hsp90 in the prostate gland epithelium. The reaction in the stroma was observed around foci of inflammation. Corpora amylacea had a rounded shape and lamellar structure, between layers of deposits of Hsp 90 was revealed. Hsp90 overexpression was found in points corpora amylacea and glandular epithelium contact. Conclusion: Overexpression of Hsp 90 in prostate tissue with CBP and corpora amylacea indicates a participation of the heat shock proteins in the development and formation of corpora amylacea. Hsp90 overexpression may be regarded as a prospective (potential) role in the corpora amylacea development.