Вивчення асоціації C+70G поліморфного сайта гена EDNRA з розвитком ішемічного атеротромботичного інсульту у курців і осіб, що не курять

Abstract

Наведено результати визначення C+70G (rs5335) поліморфізму гена рецептора ендотеліну типу А (EDNRA) у 170 хворих з ішемічним атеротромботичним інсультом (ІАТІ) і 124 осіб контрольної групи. Показано, що серед осіб, які не курять співвідношення гомозигот за основним алелем (C/C), гетерозигот (C/G) і гомозигот за мінорним алелем (G/G) у контрольній групи становило 30,1 %, 48,4 %, 21,5 %, а серед хворих з ІАТІ - 26,7 %, 55,0 %, 18,3 % відповідно (Р = 0,629, χ2 =0,927). У групі курців розподіл генотипів за вивченим поліморфізмом також достовірно не відрізнявся (25,8 %, 54,8 %, 19,4 % у контролі проти 18,0 %, 64,0 %, 18,0 % для хворих з ІАТІ; Р = 0,657, χ2 = 0,840).

Приведены результаты определения C+70G (rs5335) полиморфизма гена рецептора эндотелина типа А (EDNRA) у 170 больных с ишемическим атеротромботическим инсультом (ИАТИ) и 124 лиц контрольной группы. Показано, что среди лиц, которые не курят соотношение гомозигот по основному аллелю (C/C), гетерозигот (C/G) и гомозигот по минорному аллелю (G/G) в контрольной группе составило 30,1 %, 48,4 %, 21,5 %, а среди больных с ИАТИ – 26,7 %, 55,0 %, 18,3 % соответственно (Р = 0,629, χ2 = 0,927). В группе курильщиков распределение генотипов по изученному полиморфизму также достоверно не отличалось (25,8 %, 54,8 %, 19,4 % в контроле против 18,0 %, 64,0 %, 18,0 % для больных с ИАТИ; Р = 0,657, χ2 = 0,840).

Ischemic stroke is one of the key problems of healthcare. It is known that endothelial dysfunction (ED) plays a key role in pathogenesis of ischemic atherothrombotic stroke (IAS). Endothelin-1, a potential endogenous vasoconstrictor, is mostly responsible for development of ED. It is generally assumed that smoking is the most significant external factor that causes ED. Endothelin-1 realizes its effects through intercommunication with specific type A receptor. Therefore, endothelin type A receptor refers to key factors that influence the development of many cardiovascular diseases and ischemic stroke in particular. Purpose. To study association of C+70G polimorphic site of EDNRA gene with development of ischemic atherothrombotic stroke in smokers and non-smokers. Materials and Methods. For analysis, we used venous blood of 170 patients with IAS (42.4 % were women and 57.6 % were men) aged 40 to 85 years (average age – 64.7 ± 0.73 years) who were on the records in the outpatient department of Sumy Clinical Hospital № 5. The control group consisted of 124 patients (36.3 % were women and 63.7 % were men), average age was 76.7 ± 0.93 years. The groups were matched in sex (P = 0.294 for the χ²-test), but the average age of the first group (76.7 ± 0.93 years) was significantly higher than that of the second one (P < 0.001). Lys198Asn (rs5370) polymorphism of EDN-1 gene was determined by polymerase chain reaction, followed by restriction fragment length analysis of the allocation of them by electrophoresis in agarose gel. Statistical analysis was performed by using the software package SPSS-17. The value of P < 0.05 was considered as significant. Pathogenetic variant of stroke was determined according to the TOAST criteria. Ischemic stroke character was determined with the help of history and clinical information of the disease given by CT brain study. Results. It was demonstrated that among non-smokers, equilibration of homozygotes by basic allele (C/C), heterozygote (C/G) and homozygotes by minor allele (G/G) in control group comprised 30.1 %, 48.4 %, 21.5 %, and among IAS patients – 26.7 %, 55.0 %, 18.3 % correspondingly (Р = 0.629, χ 2 =0.927). In the group of smokers genotype distribution according to studied polymorphism also didn’t significantly differ (25.8 %, 54.8 %, 19.4 % in the control in comparison with 18.0 %, 64.0 %, 18.0 % for IAS patients; Р = 0.657, χ 2 =0.840). Conclusion. C+70G-polymorphic loci of EDNRA gene was not associated with development of IAS neither among smokers nor in the group of non-smokers.