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Title Cholinesterase in different types of the muscle tissue during the early postmortem period for diagnosis of death coming
Authors Cherkashyna, L.
Shklyar, A.
Sukhonosov, R.
Miroshnikova, O.
Naguta, L.
Olkhovskiy, V.
Demikhova, Nadiia Volodymyrivna  
Kuts, Larysa Viktorivna  
Barchan, G.
Sukhomlyn, G.
Kiptenko, Liudmyla Ivanivna  
ORCID http://orcid.org/0000-0003-4139-1645
http://orcid.org/0000-0002-9740-0718
http://orcid.org/0000-0003-4886-3342
Keywords early postmortem period
prescription of death coming
muscle tissue
cholinesterase
Type Article
Date of Issue 2021
URI https://essuir.sumdu.edu.ua/handle/123456789/83200
Publisher Bangladesh Academy of Sciences
License Creative Commons Attribution 4.0 International License
Citation Cherkashyna, L., Shklyar, A., Sukhonosov, R., Miroshnikova, O., Naguta, L., Olkhovskiy, V., Demikhova, N., Kuts, L., Barchan, G., Sukhomlyn, G., & Kyptenko, L. (2021). Cholinesterase in different types of the muscle tissue during the early postmortem period for diagnosis of death coming. Bangladesh Journal of Medical Science, 20(1), 95-100. https://doi.org/10.3329/bjms.v20i1.50352
Abstract The purpose of the research: consisted in study of postmortem regularitiesin the content of cholinesterase in different types of muscle tissue (MT) for improving accuracy of determination of the prescription of death coming PDC.Materials and methods: The activity/level of cholinesterase was determined in homogenates of the myocardial (MMH), oesophageal (OMH), diaphragm (DMH) and intercostal muscles (IMH)within the early PMP (3-13 hours after the coming of death) on 30 human corpses. MT was sampled in conditions of postmortem biopsy with use of special instruments; MT homogenates were prepared following the standard technique with subsequent determination of cholinesterase content in MT homogenates. Results and discussion: The analysis of postmortem changes in the content of cholinesterase in MT depending upon PDC revealed that after 3 hours from the moment of death coming its highest content was in muscles of the oesophagus, the least one being in MT of the intercostal muscles (respectively, (2,717.1±37.1) and (883.5±6.2) U/g, р<0.001). Levels of cholinesterase content in MT of the myocardium and diaphragm were rather close, though they differed (respectively, (1,213.8±8.8) and (1,512.8±11.5) U/g, р<0.05), and occupied an intermediate place between the corresponding values of MT of the intercostal muscles and oesophagus.A common pattern for the content of cholinesterase in different types of MT was characterized by a decrease of this content with an increase in PDC terms; besides, the dynamic lines of its changes, that we obtained, became basic ones for substantiating quantitative time dependencies and construction of relevant nomograms for forensic diagnosis of PDC by cholinesterase content in MT. Conclusions: It was proved that the content of cholinesterase in all MT homogenates, which we studied, changed regularly (and nonlinearly), but the initial and final levels of cholinesterase content differed depending upon the type of MT. Besides, the dynamics in changes of the content of cholinesterase within the time period of 3÷13 hours from the moment of death coming differed upon the type of MT too. Advantages of the technique consist in theintegrity of biochemical examination of different types of MT and simplicity in interpretation of findings. The application of the nomogram technique for assessing PDC by cholinesterase content in MT makes it possible to improve the accuracy of diagnosis for terms of the coming of death up to 60 minutes.
Appears in Collections: Наукові видання (НН МІ)

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