The Study of Association Between BGLAP HindIII-Polymorphic Variant And Type 2 Diabetes Mellitus Development Among Ukrainians With Arterial Hypertension

Abstract

Background: Nowadays, the attention of scientists is focused on the systemic energy metabolism regulation by skeleton. Bone tissue affects the glucose turnover through the production of undercarboxylated osteocalcin (ucOCN), which in turn stimulates insulin expression and secretion as well as increases sensitivity of adipocytes, muscle cells and hepatocytes for this hormone. Current evidence showed an inverse association between serum OCN concentration and adverse metabolic outcomes, assuming the crucial role of OCN in type 2 diabetes mellitus (T2DM) pathogenesis. Objective: To analyze the link between OCN gene (BGLAP) HindIII-polymorphism and T2DM occurrence among Ukrainians with arterial hypertension (AH). Methods: The study included 153 patients with T2DM (mean age ± SD 64.67±8.2 years) and 311 relatively healthy individuals (mean age 65.65±12.58 years). Polymerase chain reaction- restriction fragments length polymorphism analysis (PCR-RFLP) was performed for genotyping. Logistic regression with interaction term “genotype × AH” was used for the association analysis under four models of inheritance. Bonferroni correction was applied for accurate results. P ˂ 0.05 was considered as significant. Results: There was no statistically significant association between BGLAP HindIII-polymorphic variant under dominant, recessive, overdominant and additive models of inheritance (Pab N 0.05; Paint b N 0.05). Conclusion: No association was found between BGLAP HindIIIpolymorphic variant and T2DM development among Ukrainians with AH. Further studies are necessary to confirm the results.