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Title | Monitoring MMP-2 and MMP-7 levels in traumatic wounds of the ocular adnexa |
Authors |
Petrenko, O.V.
Dranko, M.M. Korniienko, Viktoriia Volodymyrivna Korniienko, Viktoriia Volodymyrivna Hrytsai, L.V. |
ORCID |
http://orcid.org/0000-0002-5144-2138 |
Keywords |
MMP-2 MMP-9 traumatic wounds wound healing ocular adnexa ELISA |
Type | Article |
Date of Issue | 2021 |
URI | https://essuir.sumdu.edu.ua/handle/123456789/87624 |
Publisher | Ukrainian Society of Ophthalmologists; The Filatov Institute of Eye Diseases and Tissue Therapy |
License | Creative Commons Attribution 4.0 International License |
Citation | Petrenko OV, Dranko MM, Korniienko VV, Hrytsai LV. Monitoring MMP-2 and MMP-7 levels in traumatic wounds of the ocular adnexa. J.ophthalmol.(Ukraine).2021;5:10-3. http://doi.org/10.31288/oftalmolzh202151013 |
Abstract |
Background: Matrix metalloproteinase (MMP)-2 and MMP-9 are gelatinases involved in
many physiological and pathological processes including inflammation and angiogenesis,
which make up the basis for tissue remodeling and repair. Prolonged inflammation and
delay in healing of traumatic wounds result in postoperative cicatricial deformities.
MMP-2 and MMP-9 activity monitoring might be a biomarker for healing of traumatic
ocular adnexal wounds.
Purpose: The purpose of the study was to assess MMP-2 and -9 levels in tissues in
traumatic ocular adnexal wounds at various times after trauma.
Material and Methods: We examined 60 patients presenting with traumatic ocular
adnexal wounds one hour to three months after trauma. Tissue biopsy was obtained
during surgical debridement of wounds and frozen. The main study group included
samples of traumatized ocular adnexal tissue, and the control group, samples of healthy
ocular adnexal tissue obtained during blepharoplasty. The levels of MMP-2 and MMP9 were assessed by enzyme-linked immunoassay (ELISA). The absorbance of each well
was read at a wavelength of 450 nm with a Multiskan FC plate reader, and the mean
absorbance was calculated for each reference standard and sample. Samples of patients
presenting with traumatic ocular adnexal wounds one hour to three months after trauma
were compared with controls with regard to optical density.
Results: MMP-2 and MMP-9 levels were higher in biopsy samples from traumatic
wounds than in control samples. ELISA analysis found that the peak MMP-2 expression
was observed at day 3 after trauma, and was 85% above the level in control samples.
From day 7 to day 15, this early peak fell to a level close to (15% higher than) that of
day 1. From day 15 to day 21, there was a 35 percent increase in MMP-2 expression.
MMP-9 expression levels were significantly higher than MMP-2 expression levels. The
peak MMP-9 expression was observed at day 4 after trauma, and was 155% above the
level in control samples. From day 4 to day 7, MMP-9 expression decreased to a level
85% above the level in control samples. Thereafter, MMP-9 expression increased again
to a level 100% above the level in control samples from day 8 to day 15, and gradually
decreased from day 15 to day 21.
Conclusion: Our study of MMP-2 and -9 levels in tissues in traumatic ocular adnexal
wounds at various times after trauma demonstrated that the peak MMP-2 expression was
observed at day 3, and the peak MMP-9 expression, at day 4 after trauma. The levels of
both enzymes decreased at day 7, and varied thereafter. By day 21, MMP-2 and MMP-9
levels in samples taken from the wound were as low as the levels in control samples. |
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