PWE-50 Dependence of BMI on c677t and a1298c polymorphisms of methyl entrahydrafolatreductase-gene in patients with non-alcoholic-fatty-liver-disease

Abstract

Introduction Non-alcoholic fatty liver disease (NAFLD) occurs in almost 50% of the adult population and is the cause of liver dysfunction in developed countries. The widespread use of overweight makes the problem of NAFLD relevant among both adults and children. The vast majority of patients with NAFLD are overweight and obese, which increases the risk of developing atherosclerosis, hypertension and type 2 diabetes and accelerates the progression of pathological changes in the liver. Genes associated with the onset and progression of liver disease and obesity are widely studied today. There is evidence that the methylenetetrahydrofolate reductase (MTHFR) gene affects the development and progression of NAFLD and hyperhomocysteinemia, associated with allelic polymorphism of this gene affects the development of pathological changes in the liver and in the body as a whole. Aim The aim of our study was to investigate the dependence of BMI on C677T and A1298C polymorphisms of the MTGFR gene in patients with NAFLD. Method We monitored 110 patients with NAFLD. The diagnosis of NAFLD was made on the basis of laboratory and instrumental methods of examination. The calculation of BMI was performed according to the formula Kettle - weight (kg)/height (m)2. Determination of allelic polymorphism was performed by PCR with detection of results by hybridization-fluorescence method in real time. Results As a result of our studies, we found that carriers of genotypes C677C, C677T and T677T of the MTGFR gene had BMI values of 34.7 ± 3.6, 37.9 ± 3.8 and 39.7 ± 3.2 kg/m2. We found that patients carrying the T-allele (C/T and T/T genotypes) had significantly higher BMIs compared to homozygotes for the main C-allele (C/C genotype). Studying the frequency of genotypes by A1298C polymorphism of the MTGFR gene depending on BMI, it was found that carriers of genotypes A1298A, A1298C and C1298C had BMI values of 37.1 ± 4.1, 36.6 ± 4.2 and 36.9 ± 3.8 kg/m2 in accordance. We did not find a significant difference in the frequency distribution of genotypes by A1298C polymorphism depending on BMI. Conclusions BMIs are favorably associated with the frequency of genotypes by the C677T polymorphism of the MTGFR gene and do not have such an association with the frequency of genotypes by the A1298C polymorphism of the MTGFR gene.