Романюк, Анатолій МиколайовичРоманюк, Анатолий НиколаевичRomaniuk, Anatolii MykolaiovychМоскаленко, Роман АндрійовичМоскаленко, Роман АндреевичMoskalenko, Roman AndriiovychЗакорко, Інна-Маргарита СергіївнаЗакорко, Инна-Маргарита СергеевнаZakorko, Inna-Marharyta SerhiivnaЛиндін, Микола СергійовичЛындин, Николай СергеевичLyndin, Mykola SerhiiovychПіддубний, Артем МихайловичПоддубный, Артем МихайловичPiddubnyi, Artem MykhailovychЛиндіна, Юлія МиколаївнаЛындина, Юлия НиколаевнаLyndina, Yuliia MykolaivnaРоманюк, Софія АнатоліївнаРоманюк, София АнатольевнаRomaniuk, Sofiia AnatoliivnaЯщішин, Ігор ОлександровичЯщишин, Игорь АлександровичYashchishyn, Ihor Oleksandrovych2017-12-152017-12-152017Immunohistochemical characteristics of aortic valve in the case of atherocalcinosis / A. Romaniuk, R. Moskalenko, I.-M. Zakorko [et al.] // Virchows Arch. - 2017. - Vol. 471, Suppl. 1. – S. 188.0000-0003-4385-39030000-0002-6508-01310000-0002-1691-90250000-0003-2560-13820000-0002-2132-09650000-0002-2342-0337http://essuir.sumdu.edu.ua/handle/123456789/65048Objective: According to the results of the Euro-Heart Survey on Vascular Heart Disease the most common pathology is nonrheumatic aortic steno­sis, it is also called as calcific aortic valve stenosis (CAVS), as in its pathogenesis the process of biomineralization of valve cusps and ring plays the main role Method: 30 samples of mineralized aortic valves (I group) and 10 sam­ples of aortic valve without evidence of biomineralization (II group - control) were studied. Immunohistochemical study of expression of col­lagen I, CD68, MPO, S100A9), caspase3 andosteopontin was conducted in AV tissue of both groups. Results: In CAV tissues the fibrillar component (collagen I) growths was found, but the quantitative compositions of circulating inflammatory cells (CD68+) are not significantly different from the control group. CAVs contain much more MPO+ -cells (p< 0.001) in comparison to the group of AV without biomineralization. Our data show a significant increase of the S100A9 and OPN expression in the mineralized tissue of AVs (p < 0.01). Also a higher expression level of Casp3 and MPO was found in CAVs (p< 0.05). Conclusion: High Casp 3 expression confirms the increased level o f cell elimination in the CAVs tissue, which is obviously connected with the impact of high local concentrations ofS100A9. These facts can contribute to the development of pathological biomineralization o f AV. Since osteopontin inhibits the hydroxyapatite formation by binding to the surface of the crystals, its hyperproduction is a counteracting factor against biomin­eralization in AV tissue.encneаортальний клапанаортальный клапанaortic valveбіомінералізаціябиоминерализацияbiomineralizationатерокальцинозatherocalcinosisTheses