Видання зареєстровані авторами шляхом самоархівування
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Item Effects of the sources of calcium and phosphorus on the structural and functional properties of ceramic coatings on titanium dental implants produced by plasma electrolytic oxidation(Elsevier, 2021) Кириленко, Сергій Дмитрович; Kyrylenko, Serhii Dmytrovych; Warchoł, F.; Олешко, Олександр Миколайович; Oleshko, Oleksandr Mykolaiovych; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Kazek-Kęsik, A.; Корнiєнко, Вiкторiя Володимирiвна; Korniienko, Viktoriia Volodymyrivna; Дейнека, Володимир Миколайович; Deineka, Volodymyr Mykolaiovych; Sowa, M.; Maciej, A.; Michalska, J.; Jakóbik-Kolon, A.; Matuła, I.; Basiaga, M.; Голубнича, Вікторія Миколаївна; Holubnycha, Viktoriia Mykolaivna; Stolarczyk, A.; Pisarek, M.; Mishchenko, O.; Погорєлов, Максим Володимирович; Pohorielov, Maksym Volodymyrovych; Simka, W.Plasma Electrolytic Oxidation (PEO) is as a promising technique to modify metal surfaces by application of oxide ceramic coatings with appropriate physical, chemical and biological characteristics. Therefore, objective of this research was to find the simplest settings, yet able to produce relevant bioactive implant surfaces layers on Ti implants by means of PEO. We show that an electrolyte containing potassium dihydrogen phosphate as a source of P and either calcium hydroxide or calcium formate as a source of Ca in combination with a chelating agent, ethylenediamine tetraacetic acid (EDTA), is suitable for PEO to deliver coatings with desired properties. We determined surface morphology, roughness, wettability, chemical and phase composition of titanium after the PEO process. To investigate biocompatibility and bacterial properties of the PEO oxide coatings we used microbial and cell culture tests. The electrolyte based on Ca(OH)2 and EDTA promotes active crystallization of apatites after PEO processing of the Ti implants. The PEO layers can increase electrochemical corrosion resistance. The PEO can be potentially used for development of bioactive surfaces with increased support of eukaryotic cells while inhibiting attachment and growth of bacteria without use of antibacterial agents.Item Hemostatic performance and biocompatibility of chitosan-based agents in experimental parenchymal bleeding(Elsevier, 2021) Дейнека, Володимир Миколайович; Deineka, Volodymyr Mykolaiovych; Sulaieva, O.; Пернаков, Микола Станіславович; Pernakov, Mykola Stanislavovych; Radwan-Pragłowska, J.; Janus, L.; Корнiєнко, Вiкторiя Володимирiвна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Яновська, Ганна Олександрівна; Yanovska, Hanna Oleksandrivna; Любчак, Ірина Володимирівна; Liubchak, Iryna Volodymyrivna; Юсупова, Азіза Фарходівна; Yusupova, Aziza Farkhodivna; Piątkowski, M.; Zlatska, A.; Погорєлов, Максим Володимирович; Pohorielov, Maksym VolodymyrovychThe uncontrolled parenchymatic bleeding is still a cause of serious complications in surgery and require new effective hemostatic materials. In recent years, numerous chitosan-based materials have been intensively studied for parenchymatic bleeding control but still require to increased safety and effectiveness. The current research is devoted to new hemostatic materials made of natural polymer (chitosan) developed using electrospinning and microwave-assisted methods. Hemostatic performance, biocompatibility, degradation, and in-vivo effectiveness were studied to assess functional properties of new materials. Chitosan-based agents demonstrated considerable hemostatic performance, moderate biodegradation pace and high biocompatibility in vitro. Using the electrospinning-made chitosan-copolymer significantly improved in vivo biocompatibility and degradation of Chitosan-based agents that provides opportunities for its implementation for visceral bleeding management. Chitosan aerogel could be effectively applied in hemostatic patch development due to high antibacterial activity but it is not recommended for visceral application due to moderate inflammatory effect and slow degradation.Item In vitro evaluation of electrochemically bioactivated Ti6Al4V 3D porous scaffolds(Elsevier, 2021) Myakinin, A.; Turlybekuly, A.; Погребняк, Олександр Дмитрович; Pohrebniak, Oleksandr Dmytrovych; Mirek, A.; Bechelany, M.; Любчак, Ірина Володимирівна; Liubchak, Iryna Volodymyrivna; Олешко, Олександр Миколайович; Oleshko, Oleksandr Mykolaiovych; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Корнiєнко, Вiкторiя Володимирiвна; Korniienko, Viktoriia Volodymyrivna; Leśniak-Ziółkowska, K.; Dogadkin, D.; Banasiuk, R.; Москаленко, Роман Андрійович; Moskalenko, Roman Andriiovych; Погорєлов, Максим Володимирович; Pohorielov, Maksym Volodymyrovych; Simka, W.Triply periodic minimal surfaces (TPMS) are known for their advanced mechanical properties and are wrinklefree with a smooth local topology. These surfaces provide suitable conditions for cell attachment and proliferation. In this study, the in vitro osteoinductive and antibacterial properties of scaffolds with different minimal pore diameters and architectures were investigated. For the first time, scaffolds with TPMS architecture were treated electrochemically by plasma electrolytic oxidation (PEO) with and without silver nanoparticles (AgNPs) to enhance the surface bioactivity. It was found that the scaffold architecture had a greater impact on the osteoblast cell activity than the pore size. Through control of the architecture type, the collagen production by osteoblast cells increased by 18.9% and by 43.0% in the case of additional surface PEO bioactivation. The manufactured scaffolds demonstrated an extremely low quasi-elastic modulus (comparable with trabecular and cortical bone), which was 5–10 times lower than that of bulk titanium (6.4–11.4 GPa vs 100–105 GPa). The AgNPs provided antibacterial properties against both gram-positive and gram-negative bacteria and had no significant impact on the osteoblast cell growth. Complex experimental results show the in vitro effectiveness of the PEO-modified TPMS architecture, which could positively impact the clinical applications of porous bioactive implants.Item Biological behaviour of chitosan electrospun nanofibrous membranes after different neutralisation methods(Polish Chitin Society, 2022) Корнієнко, Вікторія Володимирівна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Яновська, Ганна Олександрівна; Yanovska, Hanna Oleksandrivna; Altundal, S.; Дєдкова, Катерина Андріївна; Diedkova, Kateryna Andriivna; Самохін, Євген Олександрович; Samokhin, Yevhen Oleksandrovych; Варава, Юлія Валентинівна; Varava, Yuliia Valentynivna; Голубнича, Вікторія Миколаївна; Holubnycha, Viktoriia Mykolaivna; Viter, R.; Погорєлов, Максим Володимирович; Pohorielov, Maksym VolodymyrovychChitosan electrospun nanofibres were synthesised in two different trifluoroacetic acid (TFA)/dichloromethane (DCM) solvent ratios and then neutralised in aqueous and ethanol sodium-based solutions (NaOH and Na2CO3) to produce insoluble materials with enhanced biological properties for regenerative and tissue engineering applications. Structural, electronic, and optical properties and the swelling capacity of the prepared nanofibre membrane were studied by scanning electron microscopy, Fourier-transform infrared spectroscopy, and photoluminescence. Cell viability (with the U2OS cell line) and antibacterial properties (against Staphylococcus aureus and Escherichia coli) assays were used to assess the biomedical potential of the neutralised chitosan nanofibrous membranes. A 7:3 TFA/DCM ratio allows for an elaborate nanofibrous membrane with a more uniform fibre size distribution. Neutralisation in aqueous NaOH only maintains a partial fibrous structure. At the same time, neutralisation in NaOH ethanol-water maintains the structure during 1 month of degradation in phosphate-buffered saline and distilled water. All membranes demonstrate high biocompatibility, but neutralisation in ethanol solutions affects cell proliferation on materials made with 9:1 TFA/DCM. The prepared nanofibrous mats could constrain the growth of both gram-positive and gram-negative microorganisms, but 7:3 TFA/DCM membranes inhibited bacterial growth more efficiently. Based on structural, degradation, and biological properties, 7:3 TFA/DCM chitosan nanofibrous membranes neutralised by 70% ethanol/30% aqueous NaOH exhibit potential for biomedical and tissue engineering applications.Item Copper Nanoparticle Loaded Electrospun Patches for Infected Wound Treatment: From Development to In-Vivo Application(MDPI, 2024) Буцик, Анна Сергіївна; Butsyk, Anna Serhiivna; Варава, Юлія Валентинівна; Varava, Yuliia Valentynivna; Москаленко, Роман Андрійович; Moskalenko, Roman Andriiovych; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Піддубний, Артем Михайлович; Piddubnyi, Artem Mykhailovych; Денисенко, Анастасія Петрівна; Denysenko, Anastasiia Petrivna; Корнієнко, Валерія Олегівна; Korniienko, Valeriia Olegivna; Ramanaviciute, A.; Banasiuk, R.; Погорєлов, Максим Володимирович; Pohorielov, Maksym Volodymyrovych; Ramanavicius, A.; Корнієнко, Вікторія Володимирівна; Korniienko, Viktoriia VolodymyrivnaThis study investigates the development and application of electrospun wound dressings based on polylactic acid (PLA) nanofibers, chitosan, and copper nanoparticles (CuNPs) for the treatment of purulent skin wounds. The materials were evaluated for their structural, antibacterial, and wound healing properties using an animal model. PLA/Ch-CuNPs demonstrated the most significant antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, surpassing the other tested materials. The integration of CuNPs into the nanofiber matrices not only enhanced the antimicrobial efficacy but also maintained the structural integrity and biocompatibility of the dressings. In vivo experiments using a rat model showed that PLA/Ch-CuNPs facilitated faster wound healing with reduced exudative and inflammatory responses compared to PLA alone or PLACuNPs. Histological and immunohistochemical assessments revealed that the combination of PLA, chitosan, and CuNPs mitigated the inflammatory processes and promoted tissue regeneration more effectively. However, this study identified potential toxicity related to copper ions, emphasizing the need for careful optimization of CuNP concentrations. These findings suggest that PLA/Ch-CuNPs could serve as a potent, cost-effective wound dressing with broad-spectrum antibacterial properties, addressing the challenge of antibiotic-resistant infections and enhancing wound healing outcomes.Item From Synthesis to Clinical Trial: Novel Bioinductive Calcium Deficient HA/β-TCP Bone Grafting Nanomaterial(International and interdisciplinary scholarly open access journal, 2023) Mishchenko, O.; Яновська, Ганна Олександрівна; Yanovska, Hanna Oleksandrivna; Sulaieva, O.; Москаленко, Роман Андрійович; Moskalenko, Roman Andriiovych; Пернаков, Микола Станіславович; Pernakov, Mykola Stanislavovych; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Корнiєнко, Вiкторiя Володимирiвна; Korniienko, Viktoriia Volodymyrivna; Дейнека, Володимир Миколайович; Deineka, Volodymyr Mykolaiovych; Kosinov, O.; Varakuta, O.; Ramanavicius, S.; Varzhapetjan, S.; Ramanaviciene, A.; Krumina, D.; Knipše, G.; Ramanavicius, A.; Погорєлов, Максим Володимирович; Pohorielov, Maksym VolodymyrovychЗбільшення верхньощелепної пазухи є широко використовуваною процедурою для встановлення зубних імплантатів. Однак використання натуральних і синтетичних матеріалів у цій процедурі призвело до післяопераційних ускладнень від 12% до 38%. Щоб вирішити цю проблему, ми розробили новий наноматеріал для трансплантації кісткової тканини HA/β-TCP з дефіцитом кальцію, використовуючи метод двоетапного синтезу з відповідними структурними та хімічними параметрами для застосування підтяжки синусів. Ми продемонстрували, що наш наноматеріал демонструє високу біосумісність, посилює клітинну проліферацію та стимулює експресію колагену. Крім того, деградація β-TCP у нашому наноматеріалі сприяє утворенню тромбів, що підтримує агрегацію клітин і ріст нової кістки. У клінічному дослідженні, яке включало вісім випадків, ми спостерігали формування компактної кісткової тканини через 8 місяців після операції, що дозволило успішно встановити зубні імплантати без будь-яких ранніх післяопераційних ускладнень. Наші результати свідчать про те, що наш новий наноматеріал для пересадки кісткової тканини має потенціал для підвищення ефективності процедур збільшення гайморової пазухи.Item Fabrication and Characterization of Electrospun Chitosan/Polylactic Acid (CH/PLA) Nanofiber Scaffolds for Biomedical Application(MDPI, 2023) Самохін, Євген Олександрович; Samokhin, Yevhen Oleksandrovych; Варава, Юлія Валентинівна; Varava, Yuliia Valentynivna; Дєдкова, Катерина Андріївна; Diedkova, Kateryna Andriivna; Yanko, I.; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Radwan-Pragłowska, J.; Погорєлова, Оксана Сергіївна; Pohorielova, Oksana Serhiivna; Janus, Ł.; Погорєлов, Максим Володимирович; Pohorielov, Maksym Volodymyrovych; Корнiєнко, Вiкторiя Володимирiвна; Korniienko, Viktoriia VolodymyrivnaThe present study demonstrates a strategy for preparing porous composite fibrous materials with superior biocompatibility and antibacterial performance. The findings reveal that the incorporation of PEG into the spinning solutions significantly influences the fiber diameters, morphology, and porous area fraction. The addition of a hydrophilic homopolymer, PEG, into the Ch/PLA spinning solution enhances the hydrophilicity of the resulting materials. The hybrid fibrous materials, comprising Ch modified with PLA and PEG as a co-solvent, along with post-treatment to improve water stability, exhibit a slower rate of degradation (stable, moderate weight loss over 16 weeks) and reduced hydrophobicity (lower contact angle, reaching 21.95 ± 2.17°), rendering them promising for biomedical applications. The antibacterial activity of the membranes is evaluated against Staphylococcus aureus and Escherichia coli, with PEG-containing samples showing a twofold increase in bacterial reduction rate. In vitro cell culture studies demonstrated that PEG-containing materials promote uniform cell attachment, comparable to PEG-free nanofibers. The comprehensive evaluation of these novel materials, which exhibit improved physical, chemical, and biological properties, highlights their potential for biomedical applications in tissue engineering and regenerative medicine.Item Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers(MDPI, 2022) Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Radwan-Praglowska, J.; Голубнича, Вікторія Миколаївна; Голубничая, Виктория Николаевна; Holubnycha, Viktoriia Mykolaivna; Самохін, Євген Олександрович; Самохин, Евгений Александрович; Samokhin, Yevhen Oleksandrovych; Яновська, Ганна Олександрівна; Яновская, Анна Александровна; Yanovska, Hanna Oleksandrivna; Варава, Юлія Валентинівна; Варава, Юлия Валентиновна; Varava, Yuliia Valentynivna; Дєдкова, Катерина Андріївна; Дедкова, Екатерина Андреевна; Diedkova, Kateryna Andriivna; Janus, L.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychChitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism.Item Complementary Effect of Non-Persistent Silver Nano- Architectures and Chlorhexidine on Infected Wound Healing(MDPI, 2021) Пернаков, Микола Станіславович; Пернаков, Николай Станиславович; Pernakov, Mykola Stanislavovych; Ermini, M.L.; Sulaieva, O.; Cassano, D.; Santucci, M.; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Giannone, G.; Юсупова, Азіза Фарходівна; Юсупова, Азиза Фархадовна; Yusupova, Aziza Farkhodivna; Любчак, Ірина Володимирівна; Любчак, Ирина Владимировна; Liubchak, Iryna Volodymyrivna; Hristova, M.T.; Savchenko, A.; Голубнича, Вікторія Миколаївна; Голубничая, Виктория Николаевна; Holubnycha, Viktoriia Mykolaivna; Voliani, V.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychSurgical site infection (SSI) substantially contributes each year to patients’ morbidity and mortality, accounting for about 15% of all nosocomial infections. SSI drastically increases the rehab stint and expenses while jeopardizing health outcomes. Besides prevention, the treatment regime relies on an adequate antibiotic therapy. On the other hand, resistant bacterial strains have currently reached up to 34.3% of the total infections, and this percentage grows annually, reducing the efficacy of the common treatment schemes. Thus, new antibacterial strategies are urgently demanded. Here, we demonstrated in rats the effectiveness of non-persistent silver nano-architectures (AgNAs) in infected wound healing together with their synergistic action in combination with chlorhexidine. Besides the in vivo efficacy evaluation, we performed analysis of the bacteriological profile of purulent wound, histological evaluations, and macrophages polarization quantifications to further validate our findings and elucidate the possible mechanisms of AgNAs action on wound healing. These findings open the way for the composition of robust multifunctional nanoplatforms for the translation of safe and efficient topical treatments of SSI.Item Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials(MDPI, 2021) Дейнека, Володимир Миколайович; Дейнека, Владимир Николаевич; Deineka, Volodymyr Mykolaiovych; Sulaieva, O.; Пернаков, Микола Станіславович; Пернаков, Николай Станиславович; Pernakov, Mykola Stanislavovych; Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Яновська, Ганна Олександрівна; Яновская, Анна Александровна; Yanovska, Hanna Oleksandrivna; Юсупова, Азіза Фарходівна; Юсупова, Азиза Фархадовна; Yusupova, Aziza Farkhodivna; Ткаченко, Юлія Анатоліївна; Ткаченко, Юлия Анатольевна; Tkachenko, Yuliia Anatoliivna; Kalinkevich, O.; Zlatska, A.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychThe application of chitosan (Ch) as a promising biopolymer with hemostatic properties and high biocompatibility is limited due to its prolonged degradation time, which, in turn, slows the repair process. In the present research, we aimed to develop new technologies to reduce the biodegradation time of Ch-based materials for hemostatic application. This study was undertaken to assess the biocompatibility and hemostatic and tissue-regeneration performance of Ch-PEOcopolymer prepared by electrospinning technique. Chitosan electrospinning membranes (ChEsM) were made from Ch and polyethylene oxide (PEO) powders for rich high-porous material with sufficient hemostatic parameters. The structure, porosity, density, antibacterial properties, in vitro degradation and biocompatibility of ChEsM were evaluated and compared to the conventional Ch sponge (ChSp). In addition, the hemostatic and bioactive performance of both materials were examined in vivo, using the liver-bleeding model in rats. A penetrating punch biopsy of the left liver lobe was performed to simulate bleeding from a non-compressible irregular wound. Appropriately shaped ChSp or ChEsM were applied to tissue lesions. Electrospinning allows us to produce high-porous membranes with relevant ChSp degradation and swelling properties. Both materials demonstrated high biocompatibility and hemostatic effectiveness in vitro. However, the antibacterial properties of ChEsM were not as good when compared to the ChSp. In vivo studies confirmed superior ChEsM biocompatibility and sufficient hemostatic performance, with tight interplay with host cells and tissues. The in vivo model showed a higher biodegradation rate of ChEsM and advanced liver repair.