Видання зареєстровані авторами шляхом самоархівування
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Item Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers(MDPI, 2022) Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Radwan-Praglowska, J.; Голубнича, Вікторія Миколаївна; Голубничая, Виктория Николаевна; Holubnycha, Viktoriia Mykolaivna; Самохін, Євген Олександрович; Самохин, Евгений Александрович; Samokhin, Yevhen Oleksandrovych; Яновська, Ганна Олександрівна; Яновская, Анна Александровна; Yanovska, Hanna Oleksandrivna; Варава, Юлія Валентинівна; Варава, Юлия Валентиновна; Varava, Yuliia Valentynivna; Дєдкова, Катерина Андріївна; Дедкова, Екатерина Андреевна; Diedkova, Kateryna Andriivna; Janus, L.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychChitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism.Item Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials(MDPI, 2021) Дейнека, Володимир Миколайович; Дейнека, Владимир Николаевич; Deineka, Volodymyr Mykolaiovych; Sulaieva, O.; Пернаков, Микола Станіславович; Пернаков, Николай Станиславович; Pernakov, Mykola Stanislavovych; Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Яновська, Ганна Олександрівна; Яновская, Анна Александровна; Yanovska, Hanna Oleksandrivna; Юсупова, Азіза Фарходівна; Юсупова, Азиза Фархадовна; Yusupova, Aziza Farkhodivna; Ткаченко, Юлія Анатоліївна; Ткаченко, Юлия Анатольевна; Tkachenko, Yuliia Anatoliivna; Kalinkevich, O.; Zlatska, A.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychThe application of chitosan (Ch) as a promising biopolymer with hemostatic properties and high biocompatibility is limited due to its prolonged degradation time, which, in turn, slows the repair process. In the present research, we aimed to develop new technologies to reduce the biodegradation time of Ch-based materials for hemostatic application. This study was undertaken to assess the biocompatibility and hemostatic and tissue-regeneration performance of Ch-PEOcopolymer prepared by electrospinning technique. Chitosan electrospinning membranes (ChEsM) were made from Ch and polyethylene oxide (PEO) powders for rich high-porous material with sufficient hemostatic parameters. The structure, porosity, density, antibacterial properties, in vitro degradation and biocompatibility of ChEsM were evaluated and compared to the conventional Ch sponge (ChSp). In addition, the hemostatic and bioactive performance of both materials were examined in vivo, using the liver-bleeding model in rats. A penetrating punch biopsy of the left liver lobe was performed to simulate bleeding from a non-compressible irregular wound. Appropriately shaped ChSp or ChEsM were applied to tissue lesions. Electrospinning allows us to produce high-porous membranes with relevant ChSp degradation and swelling properties. Both materials demonstrated high biocompatibility and hemostatic effectiveness in vitro. However, the antibacterial properties of ChEsM were not as good when compared to the ChSp. In vivo studies confirmed superior ChEsM biocompatibility and sufficient hemostatic performance, with tight interplay with host cells and tissues. The in vivo model showed a higher biodegradation rate of ChEsM and advanced liver repair.