Видання зареєстровані авторами шляхом самоархівування
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Item Diversity of Peritricha (Ciliophora) in Activated Sludge Depending on the Technology of Wastewater Treatment(Polish Society of Ecological Engineering, 2024) Babko, R.; Кузьміна, Тетяна Миколаївна; Kuzmina, Tetiana Mykolaivna; Pliashechnyk, V.; Zaburko, J.; Szulżyk-Cieplak, J.; Łagód, G.The representation of Peritricha ciliates was studied in the activated sludge of two wastewater treatment plants (WWTP), one of which operates using a technology that includes alternating aerobic, anoxic and anaerobic condi tions in bioreactors (Lublin, Poland), and the other – only aerobic conditions (Sumy, Ukraine). During the study, 14 Peritricha species were identified in the WWTP Lublin, and 13 species were identified in the WWTP Sumy. The similarity of species lists was 81.5% (Sørensen index). On the basis of on the similarity and taking into account the occurrence rate (≥ 60%), a common group of Peritricha species characteristic of the activated sludge from these two types of treatment facilities was identified, which includes 4 species of colonial Peritricha: Carchesium polypinum, Epistylis coronata, Epistylis longicaudatum and Opercularia articulata, and also 4 species of solitary Peritricha: Vorticella aquadulcis, Vorticella convallaria, Vorticella infusionum and Vorticella microstoma. Despite the very high similarity in species composition, statistical analysis of the population structure of Peritricha showed a clear separation of two WWTPs with different wastewater treatment technologies. If there is a significant simi larity in the species composition of Peritricha, the species, based on their abundance and occurrence, are divided into two groups, focused on different technological schemes. The population structure of Peritricha responds to the changes in purification technology, showing the changes not so much in species composition as in their quantita tive structure. The differences in bioreactor conditions and increased effluent treatment efficiency demonstrated by the WWTP Lublin result in differences in Peritricha species structure, which are reflected in higher Peritricha abundance in WWTP Lublin compared to WWTP Sumy.Item Quantitative study of activated sludge population structure(IOP Publishing, 2021) Babko, R.; Pliashechnyk, V.; Кузьміна, Тетяна Миколаївна; Кузьмина, Татьяна Николаевна; Kuzmina, Tetiana Mykolaivna; Zaburko, J.; Danko, Y.; Łagód, G.A quantitative study of the population structure of activated sludge is an important component of biological wastewater treatment control. However, in the studying of live samples of the activated sludge, some complications arise, in particular, associated with the relatively short time of the subsample suitability. A subsample is the part of the sample that is placed on a glass slide and in which organisms are counted. The issue of optimization of counts of organisms with large amplitude of population density is considered. The results of counting ciliated protozoa in activated sludge were described. The samples were counted in 45 sub-samples of 25 µl each. An average of 10 counts was required to achieve high reliability in determining population densities with more than or equal to 1 specimen per 25 µl in sub-samples. For small population densities (less one specimen per 25 µl) of free-swimming, crawling, and sessile ciliates, 30 counts are necessary. When the density of colonial protozoan populations is established, the number of counts should be increased to 40, especially when colonies with significant differences in the number of zooids are found.Item Assessment of wastewater treatment plant effluent impact on the ecosystem of the river on the basis of the quantitative development of ciliated protozoa characteristic of the aeration tank(IWA Publishing, 2020) Babkoв, R.; Pliashechnyk, V.; Кузьміна, Тетяна Миколаївна; Кузьмина, Татьяна Николаевна; Kuzmina, Tetiana Mykolaivna; Danko, Y.; Szulzyk-Cieplak, J.; Lagod, G.The work is devoted to the task of simplifying the assessment of the effect of effluents from treatment facilities on the river hydrobiocenosis. The studies were carried out on the mountain river Uzh (Uzhgorod, Ukraine). Our approach to assessing the impact of waste treatment facilities on the river receiver is based on the estimate of the similarity of species composition and quantitative characteristics of populations of organisms from the aerotank and from the river. It is shown that the quantitative development of populations of species of ciliates from the aeration tank is a good indicator for assessing the degradation of organic matter coming with wastewater. The use of qualitative and quantitative characteristics of the protozoa from the wastewater treatment plant as a criterion for assessing the quality of the environment in the area of wastewater discharge showed their representativeness and effectiveness. The use of a limited number of species makes it possible to conduct an express assessment of the effect of effluents on receiving reservoirs for specialists working with activated sludge in the laboratories of treatment facilities.