Видання зареєстровані авторами шляхом самоархівування
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Item Microbiological aspects of the examination of the 140-year-old embalmed body of N.I. Pirogov(International Society for Plastination (ISP), 2021) Melnyk, O.P.; Ткач, Геннадій Федорович; Ткач, Геннадий Федорович; Tkach, Hennadii Fedorovych; Frišhons, J.; Guminskyi, Y.Y.; Kovalchuk, V. P.; Svoboda, J.; Melnyk, M.V.; Dzetkulicova, V.The body of N.I. Pirogov has been entombed in the Church of St. Nicolas in Vinnytsia, Ukraine, since 1881, when it was embalmed by Professor Vyvodtsev. During the re-embalming of the body of N.I. Pirogov in 2018, microbiological swabs from the body and its surroundings were taken and, among other things, skin, subcutaneous tissue, and muscle and bone tissues were taken for ultramicroscopic examination. Hyphae of fungi of the genus Penicillium citrinum were found at two sites during this examination. The occurrence of fungi can be explained by the already weakened immunity before death due to cancer, and its subsequent postmortem growth due to the non-use of formaldehyde and the placement of the body in a non-sterile and relatively humid environment of the tomb without regular control.Item Structure of the knee articular cartilage after the femur and tibia extra-articular injury(Aluna Publishing, 2021) Ткаченко, Артем Сергійович; Ткаченко, Артем Сергеевич; Tkachenko, Artem Serhiiovych; Максимова, Олена Сергіївна; Максимова, Елена Сергеевна; Maksymova, Olena Serhiivna; Кореньков, Олексій Володимирович; Кореньков, Алексей Владимирович; Korenkov, Oleksii Volodymyrovych; Возний, Андрій Петрович; Возный, Андрей Петрович; Voznyi, Andrii Petrovych; Ткач, Геннадій Федорович; Ткач, Геннадий Федорович; Tkach, Hennadii FedorovychThe aim: To study the microscopic, ultramicroscopic, and histomorphometric features of the knee articular cartilage in rats with an extra-articular injury of the femur and tibia. Materials and methods: 60 white laboratory rats divided into three groups (I – control; II – animals with traumatic femur injury; III – animals with traumatic tibia injury) were used for the study. The light microscopy was performed by Olympus BH-2 microscope (Japan), transmission electron microscopy – by JEM-1230 microscope (Japan). SPSS software (version 17.0) was used for mathematical analysis. Results: The more pronounced morphological changes were observed in the articular cartilage of the proximal tibial epiphysis after mechanical tibial injury. The thickness of the articular cartilage was 27.89 % less than in the control. The chondrocyte number in the superficial zone was lower by 8.94 %, intermediate zone – by 14.23 %, and deep zone – by 21.83%, compared to control. Herewith, the histological changes were mostly detected in the intermediate and deep zones of the articular cartilage of both bones. Also, some chondrocytes had deformed nuclei, hypertrophied organelles, numerous inclusions, and residual glycogen granules. Conclusion: The extra-articular mechanical trauma of the lower limb bones leads to pathological changes in the knee articular cartilage. The structural changes include the articular cartilage thickening, the decrease in chondrocyte number, as well as chondrocyte rearrangement due to degenerative-dystrophic processes.Item Morphological aspects of the tissues of the 140-year-old embalmed body of N.I. Pirogov(International Society for Plastination, 2021) Melnyk, O.P.; Ткач, Геннадій Федорович; Ткач, Геннадий Федорович; Tkach, Hennadii Fedorovych; Frišhons, J.; Guminskii, Y.J.; Максимова, Олена Сергіївна; Максимова, Елена Сергеевна; Maksymova, Olena Serhiivna; Dzetkuličová, V.; Melnyk, O.O.Professor Nikolay Ivanovich Pirogov (1810-1881) was an anatomist, surgeon, and scientist. He studied in Moscow and Berlin, and was one of the founders of modern surgery and aseptic procedures. He described the use of plaster for the treatment of fractures, and the use of ether as an anesthetic in combat medicine. He published a number of papers on anatomy and surgery. He died on December 5, 1881, of oral cancer. His body was embalmed by anatomist Professor David Ilyich Vyvodstev (1830- 1896), and placed in a tomb in the Church of St. Nicholas in Vinnytsia, Ukraine. The first inspection of the body was performed by a commission of experts in 1927. This was followed by several re-embalmings of the body in the 1950s and 1980s by a team led by Professor Rafail Davidovich Sinelnikov (1896-1981), and several other procedures in the 1980s and 1990s by experts from Moscow's V.I. Lenina, (now VILAR: All-Russian Research Institute of Medicinal and Aromatic Plants), until 2011. In 2017, regular care of the body was taken over by Ukrainian scientists, who, in 2018, performed all tissue and fluid analyses to determine the body’s state of preservation, and subsequent reembalmings. The results of microscopic and ultramicroscopic analysis showed some destructive changes in skin, skeletal muscle, and bone tissues. Despite these changes, however, the tissues of the body are relatively well preserved.Item Improvement of Skeletal Muscle Regeneration by Platelet-Rich Plasma in Rats with Experimental Chronic Hyperglycemia(Hindawi, 2020) Raed, Rtail; Максимова, Олена Сергіївна; Максимова, Елена Сергеевна; Maksymova, Olena Serhiivna; Ілляшенко, Вячеслав Юрійович; Ильяшенко, Вячеслав Юрьевич; Illiashenko, Viacheslav Yuriiovych; Гортинська, Олена Миколаївна; Гортинская, Елена Николаевна; Hortynska, Olena Mykolaivna; Кореньков, Олексій Володимирович; Кореньков, Алексей Владимирович; Korenkov, Oleksii Volodymyrovych; Москаленко, Павло Олександрович; Москаленко, Павел Александрович; Moskalenko, Pavlo Oleksandrovych; Mohamad, Nasser; Ткач, Геннадій Федорович; Ткач, Геннадий Федорович; Tkach, Hennadii FedorovychHerein, the structural effect of autologous platelet-rich plasma (PRP) on posttraumatic skeletal muscle regeneration in rats with chronic hyperglycemia (CH) was tested. 130 white laboratory male rats divided into four groups (I—control; II—rats with CH; III—rats with CH and PRP treatment; and IV—rats for CH confirmation) were used for the experiment. CH was simulated by streptozotocin and nicotinic acid administration. Triceps surae muscle injury was reproduced by transverse linear incision. Autologous PRP was used in order to correct the possible negative CH effect on skeletal muscle recovery. On the 28th day after the injury, the regenerating muscle fiber and blood vessel number in the CH+PRP group were higher than those in the CH rats. However, the connective tissue area in the CH group was larger than that in the CH+PRP animals. The amount of agranulocytes in the regenerating muscle of the CH rats was lower compared to that of the CH+PRP group. The histological analysis of skeletal muscle recovery in CH+PRP animals revealed more intensive neoangiogenesis compared to that in the CH group. Herewith, the massive connective tissue development and inflammation signs were observed within the skeletal muscle of CH rats. Obtained results suggest that streptozotocin-induced CH has a negative effect on posttraumatic skeletal muscle regeneration, contributing to massive connective tissue development. The autologous PRP injection promotes muscle recovery process in rats with CH, shifting it away from fibrosis toward the complete muscular organ repair.Item Morphological Characteristics and Correction of Long Tubular Bone Regeneration under Chronic Hyperglycemia Influence(Hindawi, 2020) Дудченко, Євгеній Сергійович; Дудченко, Евгений Сергеевич; Dudchenko, Yevhenii Serhiiovych; Максимова, Олена Сергіївна; Максимова, Елена Сергеевна; Maksymova, Olena Serhiivna; Pikaliuk, V.S.; Муравський, Дмитро Валерійович; Муравский, Дмитрий Валерьевич; Muravskyi, Dmytro Valeriiovych; Кіптенко, Людмила Іванівна; Киптенко, Людмила Ивановна; Kiptenko, Liudmyla Ivanivna; Ткач, Геннадій Федорович; Ткач, Геннадий Федорович; Tkach, Hennadii FedorovychIntroduction. Unsatisfactory consequences of bone regeneration disorders in diabetes mellitus (DM) patients, their high prevalence, complication number, and difficulties in treatment require further study and deeper understanding of reparative osteogenesis mechanisms under chronic hyperglycemia and finding new effective and affordable approaches to their treatment. Therefore, the aim of our work was to study the histological, ultramicroscopic, and histomorphometric features of reparative osteogenesis in rats with chronic hyperglycemia (CH), as well as to investigate the possibility of platelet-rich plasma (PRP) use in a fracture area in order to correct the negative effects of CH on reparative osteogenesis processes. Study Object and Methods. The studies were performed on 70 white laboratory rats, mature males, which were divided into the following groups: control group, animals with posttraumatic tibial defect under conditions of CH exposure, rats with experimental CH that were administered with PRP into the bone defect, and animals for the assessment of glucose homeostasis and confirmation of simulated CH. Light microscopy was performed using an Olympus BH-2 microscope (Japan). Ultramicroscopic examination was performed using REM-102 scanning electron microscope. The statistical analysis was performed using SPSS-17 software package. Results. The formation of new bone tissue in animals with CH did not occur after two weeks. Only on the 30th day of reparative osteogenesis the newly formed woven bone tissue was 61.54% of the total regenerated area. It was less than the reference value by 22.89% (P < 0:001). On the 14th day of reparative osteogenesis, the regenerated area in a group of animals with CH and PRP injection consisted of connective tissue by 68.94% (4.94% less than in animals with CH (P < 0:001)) and woven bone tissue by 31.06%, (13.51% less than in the control group (P < 0:001)). On the 30th day, the area of woven bone tissue in a regenerate of this group was less than that of the control group by 12.41% (P < 0:001). Conclusion. Thus, chronic hyperglycemia contributes to inflammation delay within the bone defect site, which makes the process of reparative osteogenesis more prolonged. The results of chronic hyperglycemia effect on bone regeneration are also impairment of osteogenic cell proliferation and shift of their differentiation towards the fibrocartilage regenerate formation. The PRP corrects the negative impact of chronic hyperglycemia on reparative osteogenesis, promoting more rapid inflammatory infiltrate removal from the bone defect site and osteogenic beam formation and remodeling of woven bone into lamellar membranous bone tissue.