Видання зареєстровані авторами шляхом самоархівування
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Item The effect of the experimental chronic hyperglycemia on the kidney and myocardium(ДУ "Інститут нефрології Національної академії медичних наук України", 2021) Yarmolenkо, Olha Serhiivna; Ярмоленкo, Ольга Сергіївна; Bumeister, Valentyna Ivanivna; Бумейстер, Валентина Іванівна; Polak,S.; Hordiienko, Olena Volodymyrivna; Гордієнко, Олена Володимирівна; Prykhodko, Olha Oleksiivna; Приходько, Ольга Олексіївна; Demikhova, Nadiia Volodymyrivna; Деміхова, Надія Володимирівна; Shkatula, Yurii Vasylovych; Шкатула, Юрій Васильович; Demikhov, Andrii Oleksiiovych; Деміхов, Андрій ОлексійовичThe number of patients with diabetes increases annually. Modern forecasts predict that diabetes will be the seventh leading cause of death in 2030. Despite many significant advances in the research of diabetes and the use of new modern treatments, the disease is still progressing, and it is necessary to continue to study the effects of diabetes on human systems and organs: kidney and myocardium.Item Рeculiarities of change in the bodyweight of rats under conditions of experimental alloxan hyperglycemia in the age aspect(Sumy State University, 2020) Dhanaraj, A.; Singh, A.Цукровий діабет був та залишається глобальною проблемою сьогодення, яка призводить до інвалідизації, втрати працездатності та смерті. Інтерес до цієї патології не згасає та спонукає науковців до пошуків нових шляхів діагностики та лікування цукрового діабету та його ускладнень. Загальновідомо, що цукровий діабет є проявом «збою» в роботі ендокринної системи, який проявляється гіперглікемією. Мета. Виявити та дослідити зміну ваги тіла щурів різних вікових категорій за умов експериментальної гіперглікемії. Матеріали та методи. Дослідження проведені на 36 білих лабораторних щурах обох статей. Піддослідні тварини були поділені на дві серії: експериментальну та інтактну. Кожна з них розбита на три групи залежно від віку щурів: молоді (2 місяці), зрілі (8 місяців) та старечі (20 місяців). Для експериментального моделювання гіперглікемії, використовували алоксану моногідрат з розрахунку 20 мг на 100 г ваги тіла щура. Визначали масу тварини, використовуючи електронні терези KERN 442-432N (Німеччина). Рівень глюкози у венозній крові щурів визначали глюкозооксидазним методом із використанням наборів реагентів «Філісіт» (Україна). Результати та обговорення. Після введення алоксану в кінці першої доби у тварин всіх вікових категорій розвинулись полідипсія, поліфагія та поліурія. Рівень глюкози у крові експериментальних молодих тварин становив 19,3±0,2 ммоль/л; у зрілих - 14,8±0,19 ммоль/л; у старечих - 22,7±0,3 ммоль/л. У тварин інтактної групи рівень глюкози в крові знаходився в межах норми, а саме: 6,3±0,2 ммоль/л; 3,5±0,1 ммоль/л; 6,5±0,24 ммоль/л, відповідно. Маса тіла інтактних щурів віком 2 місяці становила101,5±0,87 г, у тварин експериментальної серії цієї ж групи - 91,3±0,47 г. Вага тіла інтактних тварин віком 8 місяців складала 193,2±0,13 г, експериментальних – 146,6±0,3 г. Маса тіла у інтактних тварин старечого віку складала 220,2±0,19 г, у експериментальних щурів відповідного віку – 178,03±1,27 г. У відсотковому співвідношенні зменшення ваги на тлі хронічної гіперглікемії у тварин молодого віку відбулось на 11,2% (р < 0,05), у тварин зрілого віку на 31,8% (р < 0,05), у тварин старечого віку на 23,6% (р < 0,05). Висновки. В результаті розвитку алоксанової гіперглікемії у тварин молодого та старечого віку виникла гіперглікемія важкого ступеню; у щурів зрілого віку – середнього ступеню важкості. Найбільший дефіцит маси тіла виник у щурів віком 8 місяців, найменший - у щурів віком 2 місяці, що можна пояснити посиленими компенсаторними та адаптивними властивостями тварин молодого віку.Item Peculiarities of changes in the ratio of Zn / Cu in the lungs of young animals under conditions of experimental alloxan hyperglycemia(Sumy State University, 2020) Теслик, Тетяна Петрівна; Teslyk, Tetiana PetrivnaIt is generally accepted that diabetes is a multifactorial disease caused by insulin resistance and β-cell insufficiency. According to modern scientific research, each pathology has certain deviations in the elemental status. For example, in diabetes, the most common accumulation of zinc, manganese, copper, magnesium in the lungs, liver and other parenchymal organs, which is evidence of oxidative stress reactions. Aim. Detect and investigate changes in the Zn / Cu ratio in young rats under conditions of experimental hyperglycemia. Materials and methods. The studies were performed on 72 white laboratory rats of both sexes. Experimental animals were divided into two series: 1) experimental and 2) intact. Each experimental group is divided into six subgroups (6 animals) depending on the duration of the experimental study: the first - with a term of hyperglycemia 30 days, the second - 60 days, the third - 90 days, the fourth - 120 days, the fifth - 150 days, the sixth - 180 days. For experimental simulations of hyperglycemia, alloxan monohydrate was used at a rate of 20 mg per 100 g of rat body weight. Determination of Zn and Cu concentrations in μg / g was performed on an atomic absorption spectrophotometer C-115.M1 (OJSC "Selmi", Sumy, Ukraine) using a universal certified computer program "AAS SPEKTR". Results. After administration of alloxan at the end of the first day, animals of all ages developed polydipsia, polyphagia and polyuria. The level of glucose in the blood of experimental animals from 30 to 180 days ranged from 13.1 ± 0.12 to 19.3 ± 0.2 mmol / l, HbA1C - from 7.1 ± 0.05 to 9.39 ± 0.08. In animals of the intact group, the level of glucose in the blood was within normal limits (blood glucose 3.5 - 6.3 mmol / l, HbA1C 4.0 - 5.1). The ratio of Zn / Cu in intact animals aged 2 months was 8.5; 3 months - 8.5; 4 months - 8.5; 5 months - 8.53; 6 months - 8.54; 7 months - 8.56. In rats of the appropriate age on the background of hyperglycemia, the ratio of Zn / Cu for 30 days was 16.8; for 60 days - 16.95; for 90 days - 17.1; for 120 days - 17.3; for 150 days - 17.35; for 180 days - 17.3. Conclusions. The ratio of Zn / Cu increased with increasing duration of diabetes: on the 30th day compared with the control by 95.3% (p <0.05), on the 60th day - by 0.89% (p <0.05), compared with the previous month of the experiment, on the 90th day - by 0.88% (p <0.05), on the 120th day - by 0.29% (p <0.05), and on the 180th day decreased by 0.29% (p <0.05) compared with the 120th day, which indicated an increase in the intensity of antioxidant reactions with increasing duration of hyperglycemia.Item Ultrastructural changes in type 2 alveolocytes in young rats on the background of chronic hyperglycemia(Elsevier Ltd, 2023) Abdul-Rahman, T.; Awuah Wireko, A.; Теслик, Тетяна Петрівна; Teslyk, Tetiana Petrivna; Дмитрук, Сергій Миколайович; Dmytruk, Serhii Mykolaiovych; Школьна, Ірина Іванівна; Shkolna, Iryna IvanivnaDiabetes mellitus (DM) is considered as a group of metabolic diseases with a global distribution and severe complications. It is caused by insulin deficiency, which with time leads to development of pathological changes in the cardiovascular, respiratory, and other systems. Several studies have shown some features and the connection of structural changes of the lungs with DM, however very little is known regarding ultrastructural changes of type 2 alveolocytes (AT2). Materials and methods The study involved 24 white non-linear male laboratory rats which were divided into two groups (experimental and intact). The experimental group was further divided into two subgroups depending on the duration of study: the first group with hyperglycemia for 30 days, and the second with hyperglycemia for 60 days. For the experimental modeling of hyperglycemia, the rats were injected once subcutaneously with solution of alloxan monohydrate hyperglycemia. Results AT2 of the intact group had a high degree of differentiation with plates of high electron density. In AT2 of rats with hyperglycemia for 30 days, there were signs of vacuolation, mass accumulation of primary and secondary lysosomes, and lamellar bodies were grouped as conglomerates. In AT2 of the rats with 60 days of hyperglycemia, nuclei with scalloped contour, karyoplasmic outgrowths and intussusception, and condensation of heterochromatin were observed. Conclusion Under conditions of experimental chronic hyperglycemia, proliferation and destruction of AT2 are observed, which is the morphological basis for the violation of surfactant synthesis and immunocompetent properties in lung tissues of young rats.Item The remodeling of lungs under the influence of alloxan-induced hyperglycemia(Sanatatea Press Group on behalf of the Romanian Society of Diabetes Nutrition and Metabolic Diseases, 2020) Теслик, Тетяна Петрівна; Teslyk, Tetiana Petrivna; Ярмоленкo, Ольга Сергіївна; Yarmolenkо, Olha Serhiivna; Бумейстер, Валентина Іванівна; Bumeister, Valentyna Ivanivna; Деміхова, Надія Володимирівна; Demikhova, Nadiia Volodymyrivna; Приходько, Ольга Олексіївна; Prykhodko, Olha Oleksiivna; Дмитрук, Сергій Миколайович; Dmytruk, Serhii Mykolaiovych; Dehtyarova, I.Introduction: Diabetes mellitus is a global medical and social problem. 2-3% of the population suffers from diabetes mellitus. According to current forecasts, the number of people with diabetes mellitus will double every 15 years. Diabetes mellitus is the basis for the development of complex comorbidities and complications such as diabetic microangiopathy and neuropathy. Diabetes patients are at increased risk for atherosclerosis, coronary heart disease, and renal failure. Scientists around the world have been thoroughly engaged in the problem of hyperglycemia, which is the consequence of type 1 diabetes mellitus, but the causes of its occurrence have not been fully established yet. The study aimed to investigate the lungs of pre-reproductive age rats under the influence of alloxan-induced hyperglycemia. Material and Methods: 12 rats of 3 months of age were involved in the experiment. They were divided into two series: experimental and control. The Control series includes six intact rats. For the experimental modeling of hyperglycemia, we used alloxan monohydrate, pre-dissolved in 0.9% solution of sodium chloride, which was then injected intraperitoneally once at a dose of 20 mg/100 g on an empty stomach. The experiment lasted 60 days. We measured the glucose level using the Accu-Chek Advantage (Boehringer, Germany) at2, 12, and 24 hours after alloxan injection, and then weekly. The average level of glucose in the blood remained at 11 mmol/L ± 2 mmol/L. To study the remodeling of the lungs, we used the histological examination by a light microscope (Olympus BH-2) and histomorphometry using the universal certified program “SEO Scan Lab 2.0” and “SEO Image Lab 2.0”. The obtained data were processed on a personal computer using the software “GraphPad” and a licensed Excel XP spreadsheet editor (Microsoft Office 2013). The data were analyzed by unpaired t-test. P values ≤0.05 were considered as statistically significant. Results: Obtained morphometric data shows the increase of the width of alveoli by 8.5% (p < 0.05), the width of the conducting part of the respiratory bronchiole by 15.7 % (p < 0.05) and the thickness of the interalveolar septum by 8.3% (p < 0.05). The wall thickness of pulmonary vessels increased by 22.9% (p <0.05). Lymphoid nodules were more hypertrophied compared to the control ones by 2.4 times (p <0.05). Conclusions: Alloxan-induced hyperglycemia leads to the development of emphysematous changes in combination with restriction. The vascular wall of muscular pulmonary vessels was thickened due to hypertrophy of the tunica media and tunica externa. The hypertrophy of lymphoid follicles and lymph nodes of the upper and lower mediastinum was observed in experimental animals.Item Changing the chemical composition of long tubular bones of rats with diabetes mellitus type I(Sumy State University, 2018) Понирко, Аліна Олексіївна; Понырко, Алина Алексеевна; Ponyrko, Alina OleksiivnaDiabetes mellitus (diabetes mellitus) is a group of metabolic diseases is determined by hyperglycemia, which is the result wrong insulin action, or two factors together. One of the chronic complications of diabetes is diabetic osteopathy, which pronounced from moderate osteoporosis to spontaneous fractures of the tubular bones. Metabolic changes that occurs in diabetes mellitus lead to a violation of the processes of remodeling of bone tissue. In condition shortage of insulin the activity of osteoblasts decreases, and the osteoclast activity, on the contrary, increases due to this bone reabsorption intensifies. With diabetes, the processes of demineralization of bones are clearly expressed. At the diabetes, the processes of demineralization of bones are clearly expressed. Aim: The purpose:to explore the changes in the chemical composition of long tubular bones in aloxans-induced rats in native diabetic ranges.Item Biomechanical properties and macroelement element composition of long tubular bone of rats under experimental hyperglycaemia(Poltava State Medical university and Ukrainian public organization Scientific Society of Anatomists, Histologists, Embryologists and Topographoanatomists of Ukraine, 2021) Понирко, Аліна Олексіївна; Понырко, Алина Алексеевна; Ponyrko, Alina Oleksiivna; Дмитрук, Сергій Миколайович; Дмитрук, Сергей Николаевич; Dmytruk, Serhii Mykolaiovych; Бумейстер, Валентина Іванівна; Бумейстер, Валентина Ивановна; Bumeister, Valentyna IvanivnaThe pathomorphological basis for the development of diabetic disorders in various bones is a relevant subject of modern experimental research on the modeling of diabetes–associated destructive processes in the skeletal system. They are characterized by reduced bone density, which leads to an increased risk of fractures. Bone strength mainly depends on the interaction and concentration of chemical elements such as Ca, P, Mg, and K. Therefore, the purpose of our study was to determine the interdependence of changes in biomechanical parameters depending on the concentration of chemical elements in long tubular bones of rats under chronic hyperglycemia. The study was performed on 72 adult white laboratory rats lasting 180 days. It was found that starting from 30 days of uncontrolled chronic hyperglycemia, the bone mineral density in rats of the experimental group gradually decreased in comparison with the dynamics of the corresponding indexs in animals of the control group. These changes were accompanied by pronounced demineralization of both bones, as evidenced by significant losses of macronutrients (Ca, P, Mg and K). As a result, in chronic hyperglycemia gradually formed a complex violation of the structure and biomechanical properties of long tubular bones, which is a pathomorphological basis in the corresponding loci of the skeletal system.Item Additional therapy with dapagliflozin in patients with type 2 diabetes who have in adequate glycemin control(Sumy State University, 2015) Ejire, J.Study objectives: to assess the efficacy of dapagliflozin in patients with poorly controlled type 2 diabetes receiving metformin and sulfonylurea.