Видання зареєстровані авторами шляхом самоархівування
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Item Fabrication and Characterization of Electrospun Chitosan/Polylactic Acid (CH/PLA) Nanofiber Scaffolds for Biomedical Application(MDPI, 2023) Самохін, Євген Олександрович; Samokhin, Yevhen Oleksandrovych; Варава, Юлія Валентинівна; Varava, Yuliia Valentynivna; Дєдкова, Катерина Андріївна; Diedkova, Kateryna Andriivna; Yanko, I.; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Radwan-Pragłowska, J.; Погорєлова, Оксана Сергіївна; Pohorielova, Oksana Serhiivna; Janus, Ł.; Погорєлов, Максим Володимирович; Pohorielov, Maksym Volodymyrovych; Корнiєнко, Вiкторiя Володимирiвна; Korniienko, Viktoriia VolodymyrivnaThe present study demonstrates a strategy for preparing porous composite fibrous materials with superior biocompatibility and antibacterial performance. The findings reveal that the incorporation of PEG into the spinning solutions significantly influences the fiber diameters, morphology, and porous area fraction. The addition of a hydrophilic homopolymer, PEG, into the Ch/PLA spinning solution enhances the hydrophilicity of the resulting materials. The hybrid fibrous materials, comprising Ch modified with PLA and PEG as a co-solvent, along with post-treatment to improve water stability, exhibit a slower rate of degradation (stable, moderate weight loss over 16 weeks) and reduced hydrophobicity (lower contact angle, reaching 21.95 ± 2.17°), rendering them promising for biomedical applications. The antibacterial activity of the membranes is evaluated against Staphylococcus aureus and Escherichia coli, with PEG-containing samples showing a twofold increase in bacterial reduction rate. In vitro cell culture studies demonstrated that PEG-containing materials promote uniform cell attachment, comparable to PEG-free nanofibers. The comprehensive evaluation of these novel materials, which exhibit improved physical, chemical, and biological properties, highlights their potential for biomedical applications in tissue engineering and regenerative medicine.Item Impact of Electrospinning Parameters and Post-Treatment Method on Antibacterial and Antibiofilm Activity of Chitosan Nanofibers(MDPI, 2022) Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Radwan-Praglowska, J.; Голубнича, Вікторія Миколаївна; Голубничая, Виктория Николаевна; Holubnycha, Viktoriia Mykolaivna; Самохін, Євген Олександрович; Самохин, Евгений Александрович; Samokhin, Yevhen Oleksandrovych; Яновська, Ганна Олександрівна; Яновская, Анна Александровна; Yanovska, Hanna Oleksandrivna; Варава, Юлія Валентинівна; Варава, Юлия Валентиновна; Varava, Yuliia Valentynivna; Дєдкова, Катерина Андріївна; Дедкова, Екатерина Андреевна; Diedkova, Kateryna Andriivna; Janus, L.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychChitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability by nanofibers depending on the method of fabrication. The antibacterial properties of these biomaterials can be attributed to the structural modifications provided by co-solvent formulation and application of post-treatment procedure. Consequently, the proposed antimicrobial surface modification method is a promising technique to prepare biomaterials designed to induce antimicrobial resistance via antiadhesive capability and the biocide-releasing mechanism.Item Complementary Effect of Non-Persistent Silver Nano- Architectures and Chlorhexidine on Infected Wound Healing(MDPI, 2021) Пернаков, Микола Станіславович; Пернаков, Николай Станиславович; Pernakov, Mykola Stanislavovych; Ermini, M.L.; Sulaieva, O.; Cassano, D.; Santucci, M.; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Giannone, G.; Юсупова, Азіза Фарходівна; Юсупова, Азиза Фархадовна; Yusupova, Aziza Farkhodivna; Любчак, Ірина Володимирівна; Любчак, Ирина Владимировна; Liubchak, Iryna Volodymyrivna; Hristova, M.T.; Savchenko, A.; Голубнича, Вікторія Миколаївна; Голубничая, Виктория Николаевна; Holubnycha, Viktoriia Mykolaivna; Voliani, V.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychSurgical site infection (SSI) substantially contributes each year to patients’ morbidity and mortality, accounting for about 15% of all nosocomial infections. SSI drastically increases the rehab stint and expenses while jeopardizing health outcomes. Besides prevention, the treatment regime relies on an adequate antibiotic therapy. On the other hand, resistant bacterial strains have currently reached up to 34.3% of the total infections, and this percentage grows annually, reducing the efficacy of the common treatment schemes. Thus, new antibacterial strategies are urgently demanded. Here, we demonstrated in rats the effectiveness of non-persistent silver nano-architectures (AgNAs) in infected wound healing together with their synergistic action in combination with chlorhexidine. Besides the in vivo efficacy evaluation, we performed analysis of the bacteriological profile of purulent wound, histological evaluations, and macrophages polarization quantifications to further validate our findings and elucidate the possible mechanisms of AgNAs action on wound healing. These findings open the way for the composition of robust multifunctional nanoplatforms for the translation of safe and efficient topical treatments of SSI.Item Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials(MDPI, 2021) Дейнека, Володимир Миколайович; Дейнека, Владимир Николаевич; Deineka, Volodymyr Mykolaiovych; Sulaieva, O.; Пернаков, Микола Станіславович; Пернаков, Николай Станиславович; Pernakov, Mykola Stanislavovych; Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Яновська, Ганна Олександрівна; Яновская, Анна Александровна; Yanovska, Hanna Oleksandrivna; Юсупова, Азіза Фарходівна; Юсупова, Азиза Фархадовна; Yusupova, Aziza Farkhodivna; Ткаченко, Юлія Анатоліївна; Ткаченко, Юлия Анатольевна; Tkachenko, Yuliia Anatoliivna; Kalinkevich, O.; Zlatska, A.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychThe application of chitosan (Ch) as a promising biopolymer with hemostatic properties and high biocompatibility is limited due to its prolonged degradation time, which, in turn, slows the repair process. In the present research, we aimed to develop new technologies to reduce the biodegradation time of Ch-based materials for hemostatic application. This study was undertaken to assess the biocompatibility and hemostatic and tissue-regeneration performance of Ch-PEOcopolymer prepared by electrospinning technique. Chitosan electrospinning membranes (ChEsM) were made from Ch and polyethylene oxide (PEO) powders for rich high-porous material with sufficient hemostatic parameters. The structure, porosity, density, antibacterial properties, in vitro degradation and biocompatibility of ChEsM were evaluated and compared to the conventional Ch sponge (ChSp). In addition, the hemostatic and bioactive performance of both materials were examined in vivo, using the liver-bleeding model in rats. A penetrating punch biopsy of the left liver lobe was performed to simulate bleeding from a non-compressible irregular wound. Appropriately shaped ChSp or ChEsM were applied to tissue lesions. Electrospinning allows us to produce high-porous membranes with relevant ChSp degradation and swelling properties. Both materials demonstrated high biocompatibility and hemostatic effectiveness in vitro. However, the antibacterial properties of ChEsM were not as good when compared to the ChSp. In vivo studies confirmed superior ChEsM biocompatibility and sufficient hemostatic performance, with tight interplay with host cells and tissues. The in vivo model showed a higher biodegradation rate of ChEsM and advanced liver repair.Item Biocompatibility and Antibacterial Properties of ZnO-Incorporated Anodic Oxide Coatings on TiZrNb Alloy(MDPI, 2020) Олешко, Олександр Миколайович; Олешко, Александр Николаевич; Oleshko, Oleksandr Mykolaiovych; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Пшеничний, Роман Миколайович; Пшеничный, Роман Николаевич; Pshenychnyi, Roman Mykolaiovych; Варава, Юлія Валентинівна; Варава, Юлия Валентиновна; Varava, Yuliia Valentynivna; Калінкевич, Оксана Володимирівна; Калинкевич, Оксана Владимировна; Kalinkevych, Oksana Vladymyrovna; Pisarek, M.; Grundsteins, K.; Pogorielova, O.; Mishchenko, O.; Simka, W.; Вітер, Роман Віталійович; Ветер, Роман Витальевич; Viter, Roman Vitaliiovych; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychIn a present paper, we demonstrate novel approach to form ceramic coatings with incorporated ZnO nanoparticles (NPs) on low modulus TiZrNb alloy with enhanced biocompatibility and antibacterial parameters. Plasma Electrolytic Oxidation (PEO) was used to integrate ZnO nanoparticles (average size 12–27 nm), mixed with Ca(H2PO2)2 aqueous solution into low modulus TiZrNb alloy surface. The TiZrNb alloys with integrated ZnO NPs successfully showed higher surface porosity and contact angle. XPS investigations showed presence of Ca ions and absence of phosphate ions in the PEO modified layer, what explains higher values of contact angle. Cell culture experiment (U2OS type) confirmed that the surface of as formed oxide-ZnO NPs demonstrated hydrophobic properties, what can affect primary cell attachment. Further investigations showed that Ca ions in the PEO coating stimulated proliferative activity of attached cells, resulting in competitive adhesion between cells and bacteria in clinical situation. Thus, high contact angle and integrated ZnO NPs prevent bacterial adhesion and considerably enhance the antibacterial property of TiZrNb alloys. A new anodic oxide coating with ZnO NPs could be successfully used for modification of low modulus alloys to decrease post-implantation complications.Item Formation of a Bacteriostatic Surface on ZrNb Alloy via Anodization in a Solution Containing Cu Nanoparticles(MDPI, 2020) Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Олешко, Олександр Миколайович; Олешко, Александр Николаевич; Oleshko, Oleksandr Mykolaiovych; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Дейнека, Володимир Миколайович; Дейнека, Владимир Николаевич; Deineka, Volodymyr Mykolaiovych; Голубнича, Вікторія Миколаївна; Голубничая, Виктория Николаевна; Holubnycha, Viktoriia Mykolaivna; Mishchenko, O.; Kazek-Kęsik, A.; Jakobik-Kolon, A.; Пшеничний, Роман Миколайович; Пшеничный, Роман Николаевич; Pshenychnyi, Roman Mykolaiovych; Leśniak-Ziółkowska, K.; Kalinkevich, O.; Kalinkevich, A.; Pisarek, M.; Simka, W.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychHigh strength, excellent corrosion resistance, high biocompatibility, osseointegration ability, and low bacteria adhesion are critical properties of metal implants. Additionally, the implant surface plays a critical role as the cell and bacteria host, and the development of a simultaneously antibacterial and biocompatible implant is still a crucial challenge. Copper nanoparticles (CuNPs) could be a promising alternative to silver in antibacterial surface engineering due to low cell toxicity. In our study, we assessed the biocompatibility and antibacterial properties of a PEO (plasma electrolytic oxidation) coating incorporated with CuNPs (Cu nanoparticles). The structural and chemical parameters of the CuNP and PEO coating were studied with TEM/SEM (Transmission Electron Microscopy/Scanning Electron Microscopy), EDX (Energy-Dispersive X-ray Dpectroscopy), and XRD (X-ray Diffraction) methods. Cell toxicity and bacteria adhesion tests were used to prove the surface safety and antibacterial properties. We can conclude that PEO on a ZrNb alloy in Ca–P solution with CuNPs formed a stable ceramic layer incorporated with Cu nanoparticles. The new surface provided better osteoblast adhesion in all time-points compared with the nontreated metal and showed medium grade antibacterial activities. PEO at 450 V provided better antibacterial properties that are recommended for further investigation.Item In Vitro Biological Characterization of Silver-Doped Anodic Oxide Coating on Titanium(MDPI, 2020) Олешко, Олександр Миколайович; Олешко, Александр Николаевич; Oleshko, Oleksandr Mykolaiovych; Любчак, Ірина Володимирівна; Любчак, Ирина Владимировна; Liubchak, Iryna Volodymyrivna; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Юсупова, Азіза Фарходівна; Юсупова, Азиза Фархадовна; Yusupova, Aziza Farkhodivna; Олешко, Тетяна Богданівна; Олешко, Татьяна Богдановна; Oleshko, Tetiana Bohdanivna; Banasiuk, R.; Szkodo, M.; Matros-Taranets, I.; Kazek-Kęsik, A.; Simka, W.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychDespite the high biocompatibility and clinical effectiveness of Ti-based implants, surface functionalization (with complex osteointegrative/antibacterial strategies) is still required. To enhance the dental implant surface and to provide additional osteoinductive and antibacterial properties, plasma electrolytic oxidation of a pure Ti was performed using a nitrilotriacetic acid (NTA)-based Ag nanoparticles (AgNP)-loaded calcium–phosphate solution. Chemical and structural properties of the surface-modified titanium were assessed using scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) and contact angle measurement. A bacterial adhesion test and cell culture biocompatibility with collagen production were performed to evaluate biological effectiveness of the Ti after the plasma electrolytic process. The NTA-based calcium–phosphate solution with Ag nanoparticles (AgNPs) can provide formation of a thick, porous plasma electrolytic oxidation (PEO) layer enriched in silver oxide. Voltage elevation leads to increased porosity and a hydrophilic nature of the newly formed ceramic coating. The silver-enriched PEO layer exhibits an effective antibacterial effect with high biocompatibility and increased collagen production that could be an effective complex strategy for dental and orthopedic implant development.Item Тhe composite materials based on hydroxyapatite and gelatin for bone tissue application(Sumy State University, 2017) Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Яновська, Ганна Олександрівна; Яновская, Анна Александровна; Yanovska, Hanna Oleksandrivna; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym Volodymyrovych; Kosik, Ye.V.Introduction. Recent research has focused on the composites based on gelatin and carbonate containing Hydroxyapatite (HA) due to the bioactivity and biocompatibility of these materials with bone tissue. HA (Ca10 (PO4)6(OH)2 has chemical and crystallographic similarity to the carbonated apatite in human bones and is widely used for bone surgery. Gelatin (Gel) contains peptides and proteins that are excellent biocompatible and biodegradable materials for bone tissue engineering applications. Whilst many studies have investigated large concentrations of HA–Gel composites using many traditional techniques, this study investigates a combination of low powered ultrasonic irradiation and low concentration of gelatin in the co-precipitation synthesis.Item Використання скафолдів гідроксиапатит-альгінат-мідь в якості остеопластичних матеріалів(Сумський державний університет, 2016) Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Яновська, Ганна Олександрівна; Яновская, Анна Александровна; Yanovska, Hanna Oleksandrivna; Козік, Є.В.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychВступ. Одним із ключових моментів тканинної інженерії є створення скаффолдів для регенерації кісткової та хрящової тканин. Матриксні матеріали для остеорегенерації, виготовлені на основі гідроксиапатиту, викликають інтерес у зв’язку з притаманним їм властивостям біосумістності та остеокондуктивності.Item Структурированные материалы на основе гидроксиапатита и желатина для биомедицинского применения.(Институт химии поверхности им. А.А. Чуйко НАН Украины, 2015) Яновська, Ганна Олександрівна; Яновская, Анна Александровна; Yanovska, Hanna Oleksandrivna; Кузнєцов, Володимир Миколайович; Кузнецов, Владимир Николаевич; Kuznecov, Volodymur Mykolayovuch; Станиславов, А.С.; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym Volodymyrovych; Данильченко, Сергій Миколайович; Данильченко, Сергей Николаевич; Danylchenko, Serhii MykolaiovychВ даній работі одержано композитні материали гідроксиапатит-желатина (ГА–Ж) та гідроксиапатит-желатина-срібло (ГА–Ж–Ag), проведена оцінка пористості отриманих зразків та їх тестування in vivo, досліджена морфологія поверхні отриманих композитів, їх фазовий склад та особливості хімічної взаємодії між компонентами матеріалів. Основною фазою в одержаних композитах є гідроксиапатит (ГА). Аналіз результатів ІЧ-спектроскопії показав наявність взаємодії між ГА та желатиною, а також між іонами срібла та желатиною в композитних матеріалах, отриманих при спільному осадженні з водних розчинів. Отримані матеріали, що містять срібло, є біосумісними, піддаються біорезорбції та мають високий рівень пористості (75–80 %) та остеокондуктивні властивості.