Навчально-науковий медичний інститут (НН МІ)
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Item Comparative Analysis of 1470 nm and 1940 nm Wavelengths in Endovenous Laser Ablation of Large Diameter Great Saphenous Vein(Aluna Publishing, 2022) Мішура, Вікторія Вячеславівна; Мишура, Виктория Вячеславовна; Mishura, Viktoriia Viacheslavivna; Мелеховець, Юрій Володимирович; Мелеховец, Юрий Владимирович; Melekhovets, Yurii Volodymyrovych; Мелеховець, Оксана Костянтинівна; Мелеховец, Оксана Константиновна; Melekhovets, Oksana Kostiantynivna; Kovalenko, E.L.; Lihweuli, L.E.; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola SerhiiovychAim: To compare pathomorphological changes in the venous wall of large diameter great saphenous veins (GSV) after endovenous laser ablation (EVLA) using wavelengths of 1470 nm and 1940 nm. Materials and Methods: We studied 120 specimens of great saphenous veins from 30 patients with chronic venous disease with largediameter (>1 cm). Patients were randomly divided into two groups. The 1st group received EVLA using wavelengths of 1470 nm, the 2nd group received EVLA using wavelengths of 1940 nm. Four specimens were taken at the level of the lower third of the thigh after laser coagulation in each patient. Vein specimens were processed for histological studies. Both qualitative and quantitative analyses were performed to assess the degree of wall changes. Results: The share of satisfactory results when using 1470 nm laser wavelengths is 83.3%, while when using 1940 nm laser wavelengths this result is 93.3%. When using both wavelengths of laser irradiation with a GSV diameter of more than 1 cm, no unsatisfactory results are observed. Conclusions: Obtained in our study data confirmed efficacy of the 1470 and 1940 nm endovenous laser ablation in the treatment of the large size GSV (more than 10 mm). Histological exams show preferability in the 1940 nm EVLA versa 1470 nm, considering the excellent result in the uniformity of distribution and safety in the deepness of the thermal injury.Item Reparative processes features in trophic ulcers caused by diabetes mellitus with the use of platelet-rich plasma(ALUNA, 2020) Дужий, Ігор Дмитрович; Дужий, Игорь Дмитриевич; Duzhyi, Ihor Dmytrovych; Ніколаєнко, Андрій Сергійович; Николаенко, Андрей Сергеевич; Nikolaienko, Andrii Serhiiovych; Попадинець, Василь Миронович; Попадинець, Василий Миронович; Popadynets, Vasyl Myronovych; Кравець, Олександр Валерійович; Кравец, Александр Валерьевич; Kravets, Oleksandr Valeriiovych; Гресько, Ігор Яремович; Гресько, Игорь Еремеевич; Hresko, Ihor Yaremovych; Голубничий, Станіслав Олександрович; Голубничий, Станислав Александрович; Holubnychyi, Stanislav Oleksandrovych; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii MykolaiovychThe improvement of the treatment results of the lower limbs ulcers, caused by the diabetes mellitus by using our technique of the platelet-rich plasma application; the study of the features of the morphological and immunohistochemical changes, and the effect of the growth factors of the platelet-rich plasma on the regeneration and healing of the ulcers. 38 patients with the trophic ulcers of the lower limbs, caused by diabetes mellitus were involved in the study. To assess the morphological features of the reparative processes before and after the treatment with PRP, the histological and immunohystochemical studies of the biopsy specimen of ulcers were carried out.Item Fluorophore-conjugated Helicobacter pylori recombinant membrane protein (HopQ) labels primary colon cancer and metastases in orthotopic mouse models by binding CEA-related cell adhesion molecules(Elsevier Inc. on behalf of Neoplasia Press, Inc., 2020) Hollandsworth, H.M.; Schmitt, V.; Amirfakhri, S.; Filemoni, F.; Schmidt, A.; Landström, М.; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Backert, S.; Gerhard, M.; Wennemuth, G.; Hoffman, R.M.; Singer, B.B.; Bouvet, M.HopQ is an outer-membrane protein of Helicobacter pylori that binds to human carcinoembryonic antigen-related celladhesion molecules (CEACAMs) with high specificity. We aimed to investigate fluorescence targeting of CEACAMexpressing colorectal tumors in patient-derived orthotopic xenograft (PDOX) models with fluorescently labeled recombinant HopQ (rHopQ). Western blotting, flow cytometry and ELISA were performed to determine the efficiency of rHopQ binding to CEACAMs. rHopQ was conjugated to IR800DyeCW (rHopQ IR800). Nude mice received orthotopic implantation of colon cancer tumors. Three weeks later, mice were administered 25 μg or 50 μg HopQ-IR800 and imaged 24 or 48 h later. Intravital images were analyzed for tumor-to-background ratio (TBR). Flow cytometry and ELISA demonstrated binding of HopQ to CEACAM1, 3 and 5. Dose-response intravital imaging in PDOX models demonstrated optimal results 48 h after administration of 50 μg rHopQ-IR800 (TBR = 3.576) in our protocol. Orthotopic models demonstrated clear tumor margins of primary tumors and small regional metastases with a mean TBR = 3.678 (SD ± 1.027). rHopQ showed specific binding to various CEACAMs in PDOX models. rHopQ may be useful for CEACAM-positive tumor and metastasis detection for pre-surgical diagnosis, intra-operative imaging and fluorescence-guided surgery.