Навчально-науковий медичний інститут (НН МІ)
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Item Glucoraphanin Triggers Rapid Antidepressant Responses in a Rat Model of Beta Amyloid-Induced Depressive-like Behaviour(MDPI, 2022) Tucci, P.; Bove, M.; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Dimonte, S.; Morgese, M.G.; Schiavone, S.; Di Cesare Mannelli, L.; Ghelardini, C.; Trabace, L.Glucoraphanin (GRA) is a natural compound that has shown beneficial effects in chronic diseases and in central nervous system disorders. Moreover, GRA displayed antidepressant activity in preclinical models. We have previously demonstrated that a single intracerebroventricular administration of soluble amyloid-beta 1-42 (sAβ 1-42) in rat evokes a depressive-like phenotype by increasing immobility frequency in the forced swimming test (FST). The aim of this work was to investigate the effect of GRA in naïve and in sAβ-1-42-treated rats by using the FST. Behavioural analyses were accompanied by neurochemical and biochemical measurements in the prefrontal cortex (PFC), such as serotonin (5-HT), noradrenaline (NA), kynurenine (KYN), tryptophan (TRP), reactive oxygen species (ROS) and the transcription nuclear factor kappa B (NF-kB) levels. We reported that GRA administration in naïve rats at the dose of 50 mg/kg reduced the immobility frequency in the FST and increased 5-HT and NA levels in the PFC compared to controls. At the same dose, GRA reverted depressive-like effects of sAβ 1-42 administration, restored the 5-HT levels and reduced NF-kB, KYN and ROS levels in PFC. In conclusion, GRA rapidly reverting depressive-like behaviour, together with biochemical and neurochemical alterations, might represent a safe and natural candidate for the treatment of depression.Item Precision Medicine in Alzheimer’s Disease: Investigating Comorbid Common Biological Substrates in the Rat Model of Amyloid Beta-Induced Toxicity(Frontiers Media S.A., 2022) Morgese, M.G.; Bove, M.; Di Cesare Mannell, L.; Schiaone, S.; Coli, A.L.; Dimonte, S.; Mhillaj, E.; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Tucci, P.; Ghelardini, C.; Traace, L.Alzheimer’s disease (AD), one of the most widespread neurodegenerative disorder, is a fatal global burden for the elder population. Although many efforts have been made, the search of a curative therapy is still ongoing. Individuating phenotypic traits that might help in investigating treatment response is of growing interest in AD research. AD is a complex pathology characterized by many comorbidities, such as depression and increased susceptibility to pain perception, leading to postulate that these conditions may rely on common biological substrates yet to be determined. In order to investigate those biological determinants to be associable with phenotypic traits, we used the rat model of amyloid beta-induced toxicity. This established model of early phase of AD is obtained by the intracerebroventricular injection of soluble amyloid beta1-42 (Aβ) peptide 7 days before performing experiments. In this model, we have previously reported increased immobility in the forced swimming test, reduced cortical serotonin levels and subtle alterations in the cognitive domain a depressive-like phenotype associated with subtle alteration in memory processes. In light of evaluating pain perception in this animal model, we performed two different behavioral tests commonly used, such as the paw pressure test and the cold plate test, to analyze mechanical hyperalgesia and thermal allodynia, respectively. Behavioural outcomes confirmed the memory impairment in the social recognition test and, compared to sham, Aβ-injected rats showed an increased selective susceptibility to mechanical but not to thermal stimulus. Behavioural data were then corroborated by neurochemical and biochemical biomarker analyses either at central or peripheral level. Data showed that the peptide injection evoked a significant increase in hypothalamic glutamate, kynurenine and dopamine content, while serotonin levels were reduced. Plasma Cystatin-C, a cysteine protease, was increased while serotonin and melatonin levels were decreased in Aβ-injected rats. Urinary levels paralleled plasma quantifications, indicating that Aβ-induced deficits in pain perception, mood and cognitive domain may also depend on these biomarkers. In conclusion, in the present study, we demonstrated that this animal model can mimic several comorbid conditions typical of the early phase of AD. Therefore, in the perspective of generating novel therapeutic strategies relevant to precision medicine in AD, this animal model and the biomarkers evaluated herein may represent an advantageous approach.Item Precision Medicine in Alzheimer’s Disease: Investigating Comorbid Common Biological Substrates in the Rat Model of Amyloid Beta-Induced Toxicity(Frontiers Media S.A., 2021) Morgese, M.G.; Bove, M.; Di Cesare Mannelli, L.; Schiavone, S.; Colia, A.L.; Dimonte, S.; Mhillaj, E.; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Tucci, P.; Ghelardini, C.; Trabace, L.Alzheimer’s disease (AD), one of the most widespread neurodegenerative disorder, is a fatal global burden for the elder population. Although many efforts have been made, the search of a curative therapy is still ongoing. Individuating phenotypic traits that might help in investigating treatment response is of growing interest in AD research. AD is a complex pathology characterized by many comorbidities, such as depression and increased susceptibility to pain perception, leading to postulate that these conditions may rely on common biological substrates yet to be determined. In order to investigate those biological determinants to be associable with phenotypic traits, we used the rat model of amyloid beta-induced toxicity. This established model of early phase of AD is obtained by the intracerebroventricular injection of soluble amyloid beta1-42 (Aβ) peptide 7 days before performing experiments. In this model, we have previously reported increased immobility in the forced swimming test, reduced cortical serotonin levels and subtle alterations in the cognitive domain a depressive-like phenotype associated with subtle alteration in memory processes. In light of evaluating pain perception in this animal model, we performed two different behavioral tests commonly used, such as the paw pressure test and the cold plate test, to analyze mechanical hyperalgesia and thermal allodynia, respectively. Behavioural outcomes confirmed the memory impairment in the social recognition test and, compared to sham, Aβ-injected rats showed an increased selective susceptibility to mechanical but not to thermal stimulus. Behavioural data were then corroborated by neurochemical and biochemical biomarker analyses either at central or peripheral level. Data showed that the peptide injection evoked a significant increase in hypothalamic glutamate, kynurenine and dopamine content, while serotonin levels were reduced. Plasma Cystatin-C, a cysteine protease, was increased while serotonin and melatonin levels were decreased in Aβ-injected rats. Urinary levels paralleled plasma quantifications, indicating that Aβ-induced deficits in pain perception, mood and cognitive domain may also depend on these biomarkers. In conclusion, in the present study, we demonstrated that this animal model can mimic several comorbid conditions typical of the early phase of AD. Therefore, in the perspective of generating novel therapeutic strategies relevant to precision medicine in AD, this animal model and the biomarkers evaluated herein may represent an advantageous approach.Item Проблеми діагностики первинного раку маткових труб під час і після операції(Видавничий дім «Триліст», 2021) Сумцов, Дмитро Георгійович; Сумцов, Дмитрий Георгиевич; Sumtsov, Dmytro Heorhiiovych; Гладчук, І.З.; Сумцов, Георгій Олексійович; Сумцов, Георгий Алексеевич; Sumtsov, Heorhii Oleksiiovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Запорожан, В.М.До теперішнього часу при первинному раку маткових труб (РМТ) достовірні діагнози до операції коливаються від 0% до 10–15%. Навіть під час операцій число помилкових діагнозів досягає 30–50%, оскільки РМТ часто маскується під невинний гідро-гематосальпінкс або інші захворювання.Мета дослідження: вивчити можливості діагностики РМТ при лапаротомії та лапароскопії, макроскопічному дослідженні видалених макропрепаратів, субопераційному застосуванні морфологічних досліджень, дослідити проблеми гістологічної інтерпретації пухлини після операції.Матеріали і методи. За період з 1966 по 2020 роки авторами статті вибрані і ретроспективно вивчені досить інформативні історії хвороби 105 хворих на РМТ у віці від 34 до 78 років (середній вік – 55,8 років). Всі хворі прооперовані. Під час операцій проведені ревізії органів таза і черевної порожнини. Видалені макропрепарати досліджені макроскопічно і гістологічно. За показаннями під час операцій застосовані гістологічні і цитологічні методи експрес-діагностики. Для інтерпретації рідкісних і важких для діагностики пухлин після операцій застосовані імуногістохімічні методи дослідження.Ре зу л ьта т и. Тільки в 7 (6,6%) з 105 хворих на РМТ пухлину під час операції не було розпізнано, а у 18 (17,1%) діагноз встановлений лише після субопераційного гістологічного дослідження. Помилки під час операцій при початкових формах РМТ (без ретельного вивчення макропрепаратів і субопераційного морфологічного дослідження) можуть досягати 23,8%. Проблеми з інтерпретацією первинності ураження, які виникли в 11 (10,5%) хворих з вираженим пухлинним процесом, не завадили вибору адекватної операції. Поширення пухлини за межі маткової труби виявлено у 69 (72,4%) пацієнток, у 25 (26,2%) з них – з метастазами в лімфатичні вузли.Висновок. Ретельна ревізія органів черевної порожнини і макроскопічне обстеження видалених препаратів із застосуванням субопераційних морфологічних досліджень дозволяє уникнути помилок в діагностиці та хірургічному лікуванні хворих на РМТ.Item Morphological Peculiarities of Parasitic (Trichosomoides crassicauda) Infection in Rat Urinary Bladder(Sciendo, 2021) Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Гирявенко, Наталія Іванівна; Гирявенко, Наталья Ивановна; Hyriavenko, Nataliia Ivanivna; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Линдіна, Юлія Миколаївна; Лындина, Юлия Николаевна; Lyndina, Yuliia Mykolaivna; Сікора, Катерина Олексіївна; Сикора, Екатерина Алексеевна; Sikora, Kateryna Oleksiivna; Чижма, Руслана Анатоліївна; Чижма, Руслана Анатольевна; Chyzhma, Ruslana Anatoliivna; Дяченко, Олена Олегівна; Дяченко, Елена Олеговна; Diachenko, Olena Olehivna; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii MykolaiovychTrichosomoides crassicauda (T. crassicauda) is a parasite commonly localized in the urinary bladder (UB) of laboratory and wild rats. The presence of these helminths can influence the prediction of pathological changes in the UB. Therefore, the purpose of this research was to make a comprehensive study of the features of the morphological changes in the UB wall of white laboratory rats as a result of T. crassicauda infestation. The study was performed on male rats using histological (Hematoxyline-Eosin and Alcian Blue staining) and immunohistochemical (Ki-67, Hsp70, Hsp90α, CD3 and CD20) methods. T. crassicauda was detected in both urine and UB samples. Morphological changes were observed as disruption in urothelial cell stratification and insignificant proliferative and immune responses in the UB. Increased heat shock protein levels were observed which may suggest a natural body’s resistance to this parasite.Item The role of psammoma bodies in the ovarian serous adenocarcinoma(Sumy State University, 2020) Chizhma, R.A.; Soloviov N.O.; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Піддубний, Артем Михайлович; Поддубный, Артем Михайлович; Piddubnyi, Artem MykhailovychOne of the current problems among women’s reproductive system diseases is ovarian tumors. Each year the risk of this disease shows a tendency to increase. The pathognomonic feature of the morphological diagnostic of ovarian cancer is Psammoma bodies (PBs), which are represented by plasticized calcium structures and are placed in the form of concentration circles. Should be noted, that the process of pathological biomineralization is most common in serous papillary carcinoma, but the mechanism of PBs formation is not fully understood yet.Item Effect of heavy metals on microstructural and microelemental changes in the rats urinary bladder(Springer, 2020) Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Гирявенко, Наталія Іванівна; Гирявенко, Наталья Ивановна; Hyriavenko, Nataliia Ivanivna; Линдіна, Юлія Миколаївна; Лындина, Юлия Николаевна; Lyndina, Yuliia Mykolaivna; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Піддубний, Артем Михайлович; Поддубный, Артем Михайлович; Piddubnyi, Artem Mykhailovych; Сікора, Катерина Олексіївна; Сикора, Екатерина Алексеевна; Sikora, Kateryna Oleksiivna; Kuts, V.Global environmental pollution leads to the distribution of heavy metal salts (HMS) in the ecosystem and their impact on the organism. To study the influence of the heavy metals (HM) accumulation on the ultrastructural changes in rat’s urinary bladder (UB).Item The study of the toxic effect of the heavy metals salts on the erythropoiesis in the rats(Springer, 2020) Линдіна, Юлія Миколаївна; Лындина, Юлия Николаевна; Lyndina, Yuliia Mykolaivna; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Soroka, Y.; Romaniuk, S.; Карпенко, Людмила Іванівна; Карпенко, Людмила Ивановна; Karpenko, Liudmyla Ivanivna; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii MykolaiovychHeavy metals salts (HMS) are the most common pollutants that are proved to have the negative effect. The objective is to determine the morphological features of the marrow in rats, caused by the combined effect of the heavy metals salts.Item Ultraviolet impact on rat skin(Springer, 2020) Сулим, Григорій Анатолійович; Сулим, Григорий Анатольевич; Sulym, Hryhorii Anatoliiovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Сікора, Катерина Олексіївна; Сикора, Екатерина Алексеевна; Sikora, Kateryna Oleksiivna; Гирявенко, Наталія Іванівна; Гирявенко, Наталья Ивановна; Hyriavenko, Nataliia Ivanivna; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii MykolaiovychThe aim of our study was to establish a >model imitating of UV-B (wavelength peak is 311 nm) therapy for rat’s skin and to find match macroscopical and following histological skin changes including melanocytes spreading and melanin distribution.Item CEACAM1 expression in the normal uterus of rats(Springer, 2020) Сікора, Катерина Олексіївна; Сикора, Екатерина Алексеевна; Sikora, Kateryna Oleksiivna; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Кравцова, Ольга Іванівна; Кравцова, Ольга Ивановна; Kravtsova, Olha Ivanivna; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Singer, B.B.The carcinoembryonic antigen-related adhesion molecules (CEACAM) play a significant role in numerous physiological processes, such as cell-cell and cell-ECMadhesion, angiogenesis, proliferation, etc. The objective is characterization of the CEACAM1 expression pattern in normal tissues of rat uterus.