Аналіз асоціації поліморфних варіантів Bsml, Apai, Taql гена VDR із розвитком хронічного генералізованого пародонтиту

Abstract

Наведено результати вивчення BsmI, ApaI, TaqI-поліморфізмів гена VDR у 116 хворих iз хронічним генералізованим пародонтитом та 67 осіб контрольної групи. Встановлено, що BsmI, ApaI, TaqI-поліморфні варіанти гена VDR асоційовані з розвитком хронічного пародонтиту в українській популяції. У гомозигот за мінорним алелем B/B (BsmI-поліморфізм) ризик настання хронічного генералізованого пародонтиту в 3,14 рази, гетерозигот a/A (ApaI-поліморфізм) – 2,21 рази і T/t (TaqI-поліморфізм) – 2,08 рази більший, ніж у гомозигот за основним алелем

Приведены результаты изучения BsmI, ApaI, TaqI-полиморфизмов гена VDR у 116 пациентов с хроническим генерализованным пародонтитом и 67 лиц контрольной группы. Установлено, что BsmI, ApaI, TaqI-полиморфные варианты гена VDR ассоциированы с развитием хронического пародонтита в украинской популяции. У гомозигот по минорному аллелю B/B (BsmI-полиморфизм) риск возникновения хронического генеразиванного пародонтита в 3,14 раза, гетерозигот a/A (ApaI-полиморфизм) – 2,21 раза и T/t (TaqI-полиморфизм) – 2,08 раза выше, чем у гомозигот по основному аллелю.

Introduction. Nowadays the role of vitamin D and vitamin D receptor (VDR) in the development of common multifactor diseases, including diabetes mellitus, multiple sclerosis, cancer of various localizations, arterial hypertension, ischemic stroke, skin diseases etc. is actively studied. Participation of vitamin D in bone remodeling and mineralization processes, as well as its importance in the inflammatory reactions and immune response regulation, determine its important role in the pathogenesis of periodontitis. Since the mineral density reducing of the bone tissue of the jaw alveolar processes is a favorable background for the detrimental effect of periodontopathogenic microflora, the question about association between periodontitis and mineral metabolism regulation disorders attracts the attention of dentists more and more. The problem of VDR gene polymorphisms influence on the probability of the periodontitis onset and progression is also in the range of studied issues. In recent years a lot of studies have been conducted in different countries but their results are quite controversial. Purpose. To analyze the association between VDR gene BsmI, ApaI, TaqI polymorphisms and сhronic generalized periodontitis development. Materials and Methods. The buccal epithelial cells of 116 patients with сhronic generalized periodontitis and 67 control subjects were used. VDR gene BsmI (rs1544410) ApaI (rs7975232) and TaqI (rs731236) (rs9934438) polymorphisms genotyping was performed using PCR-RFLP (polymerase chain reaction with following restriction fragment length polymorphism analysis) techniques. Statistical analyses were performed using Statistical Package for Social Science software (SPSS, version 17.0, Chicago, IL, USA). All statistical tests were two-sided, the value of P < 0.05 was considered as significant. Results. The data obtained in present work demonstrated that distribution of VDR gene BsmI, but not ApaI and TaqI polymrophisms genotypes was statistically different between periodontitis patients and control group (P = 0.046, P = 0.084, P = 0.085 resp.). Herewith logistic regression analysis revealed the risk for chronic generalized periodontitis development in patients with BB genotype (BsmI polymorphism) was higher than for individuals with bb genotype (OR = 3.143; Р = 0.009). Heterozygotes aA (ApaI polymorphism) had a higher risk for chronic periodontitis than aa-genotype carriers (OR = 2.208; P = 0.029). It has been also shown that probability of chronic periodontitis development in patients with Tt-genotype (TaqI polymorphism) was higher compared to subjects with TT-genotype (OR = 2.082; P = 0.028).Conclusion. VDR gene BsmI, ApaI, TaqI polymorphisms are associated with сhronic generalized periodontitis development in Ukrainian population. BB genotype (BsmI polymorphism), aA genotype (ApaI polymorphism) and Tt genotype (TaqI polymorphis) are related to increased risk for chronic generalized periodontitis compared with main allele homozygotes.