|Title||Immunohistochemical characteristics of aortic valve in the case of atherocalcinosis|
Romaniuk, Anatolii Mykolaiovych
Moskalenko, Roman Andriiovych
Zakorko, Inna-Marharyta Serhiivna
Lyndin, Mykola Serhiiovych
Piddubnyi, Artem Mykhailovych
Lyndina, Yuliia Mykolaivna
Романюк, Софія Анатоліївна
Романюк, София Анатолиевна
Romaniuk, Sofia Anatoliyivna
Yashchishyn, Ihor Oleksandrovych
|Date of Issue||2017|
|Citation||Immunohistochemical characteristics of aortic valve in the case of atherocalcinosis / A. Romaniuk, R. Moskalenko, I.-M. Zakorko [et al.] // Virchows Arch. - 2017. - Vol. 471, Suppl. 1. – S. 188.|
Objective: According to the results of the Euro-Heart Survey on Vascular Heart Disease the most common pathology is nonrheumatic aortic stenosis, it is also called as calcific aortic valve stenosis (CAVS), as in its pathogenesis the process of biomineralization of valve cusps and ring plays the main role Method: 30 samples of mineralized aortic valves (I group) and 10 samples of aortic valve without evidence of biomineralization (II group - control) were studied. Immunohistochemical study of expression of collagen I, CD68, MPO, S100A9), caspase3 andosteopontin was conducted in AV tissue of both groups.
Results: In CAV tissues the fibrillar component (collagen I) growths was found, but the quantitative compositions of circulating inflammatory cells (CD68+) are not significantly different from the control group. CAVs contain much more MPO+ -cells (p< 0.001) in comparison to the group of AV without biomineralization. Our data show a significant increase of the S100A9 and OPN expression in the mineralized tissue of AVs (p < 0.01). Also a higher expression level of Casp3 and MPO was found in CAVs (p< 0.05).
Conclusion: High Casp 3 expression confirms the increased level o f cell elimination in the CAVs tissue, which is obviously connected with the impact of high local concentrations ofS100A9. These facts can contribute to the development of pathological biomineralization o f AV. Since osteopontin inhibits the hydroxyapatite formation by binding to the surface of the crystals, its hyperproduction is a counteracting factor against biomineralization in AV tissue.
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