|Title||The effect of prostatic stones on the immunophenotype of prostate cancer cells|
Piddubnyi, Artem Mykhailovych
Lyndin, Mykola Serhiiovych
Moskalenko, Yuliia Vasylivna
Vynnychenko, Ihor Oleksandrovych
Romaniuk, Anatolii Mykolaiovych
Moskalenko, Roman Andriiovych
|Date of Issue||2019|
|Publisher||Lithuanian University of Health Sciences|
|Citation||The effect of prostatic stones on the immunophenotype of prostate cancer cells / A. Piddubnyi, M. Lyndin, Y. Moskalenko [et al.] // Medicina: 77th International Scientific Conference of the University of Latvia (February 22, 2019 Riga, Latvia). - 2019. - Vol. 55. - Supplement 1. - P. 95.|
Background.Prostate cancer (PGC) is leading the way in the structure of cancer morbidity
around the world. The presence of prostatic concretions (PC) and skeletal metastases significantly
reduces the patients’ life quality and worsens the prognosis of the disease. However, the phenomenon of osteotropic tumor metastases is not sufficiently studied.
Aim.To define the impact of PC on the development of prostate cancer metastasis to the bones.
Methods.10 samples of PGC with PC, 10 intact PGC specimens, and PGC metastasis tissue to
lymph node were analysed. Histology (staining with hematoxylin-eosin), histochemistry (von Kossa reaction, alizarin red), immunohistochemistry (OSN, Col I, Col II, Bax, Casp3, MPO, CD68),
Results.All PC samples had the calcium hydroxyapatite origin with a slight deviation from the
stoichiometric composition and admixture of extraneous elements. Assessment of apoptotic potential (Вах and Саsp3) and inflammatory infiltration (MPO and CD68) revealed the significantly
higher rates of these processes in PGC tissue with PC (p <0.01). We suggest that the combined
effect of these factors predetermines the development of a specific phenotype of cancer cells. This
phenomena is manifested by the significantly higher OSN and Col I osteoblastic markers expression in PGC with PC and metastatic tissue (p <0.05).
Conclusions.The determination of osteoblastic markers expression in PGC can be used as criterion for evaluation of probability of bone metastases development. The presence of PC in PGC
tissue can be an important prognostic information for clinicians.The presence of these pathological inclusions, namely the PC, promotes the expression of osteoblastic markers in PGC tissue, which helps the tumor cells to recognize a favorable microenvironment.
Acknowlegments.This research has been performed with the financial support of grants of the Ministry of Education and Science of Ukraine No. 0117U003937 “The development of tumor diagnosis method of reproductive system organs using cellular adhesion molecules of cancerembryonic antigen” and No. 0118U003570 “The efficiency of “liquid biopsy” and tissue biopsy in the diagnosis and treatment of malignant tumors”.
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