Наукові видання (НН МІ)
Permanent URI for this collectionhttps://devessuir.sumdu.edu.ua/handle/123456789/132
Browse
3 results
Search Results
Item Histological and immunohistochemical features of medullary breast cancer(Uniwersytet Jagielloński – Collegium Medicum, 2015) Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Линдіна, Юлія Миколаївна; Лындина, Юлия Николаевна; Lyndina, Yuliia Mykolaivna; Panasovska, K.O.Disputes take place among many scientists about the position of MC in the classification of breast cancer. Some say that this kind of tumor is a sign of invasive ductal carcinoma (IDC). Instead, most of modern researchers distinguish MC of the breast as a separate nosological unit. Primarily there were selected 20 cases of MC and 10 cases of IDC (as control group). The immunohistochemical study revealed the presence of ER, PR, HER2/neo, p53, Ki-67, MMP1 and E-cadherin receptors. In the study of receptor status of tumors it was observed that 100% of MC cases were estrogen-, progesterone- and HER2/neu negative. The status of tumors on receptor p53 and Ki-67 was as follows: p53+ status had 80% and Ki-67+ had 85% of tissues of MC. In 75% of cases MC cells expressed marker of adhesion and in 100% of cases cells were receptor-negative for expression of MMP1. The data of the study show that the invasive ductal carcinoma and medullary carcinoma are completely independent and different types of malignancy in the breast. The favorable behavior of medullary cancer is associated with expression of E-cadherin receptors, which are essentially adhesion factor and absence of MMP1 which are factors of metastatic potential of the tumor.Item The role of heavy metal salts in pathological biomineralization of breast cancer tissue(Wroclaw Medical University, 2016) Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Гортинська, Олена Миколаївна; Гортинская, Елена Николаевна; Hortynska, Olena Mykolaivna; Линдіна, Юлія Миколаївна; Лындина, Юлия Николаевна; Lyndina, Yuliia MykolaivnaBackground. The process of pathological biomineralization plays an important part in the morphogenesis of tumors. The role of heavy metal salts in the pathological mineralization of breast cancer tissue should not be ruled out, considering their ability to enter into covalent bonds with calcium salt molecules. Objectives. The aim of the study was to investigate the microelement composition of breast cancer calcifications and the participation of heavy metals in their formation process. Material and Methods. The material for the study consisted of 20 specimens of breast cancer tissue in which calcifications had been found in histological tests (hematoxylin-eozin and alizarin red S staining). The chemical composition of the calcifications was studied using a scanning electron microscope with an energy-dispersive spectrometer. Results. Alizarin red S staining detected the presence of concrements in tumor tissue and rings of calcification around these deposits. Examining the biomineralization with energy dispersive spectrometry showed that along with calcium and phosphorus, it contained microelements such as iron, zinc, copper, chromium and nickel, which can replace calcium ions in the exterior part of hydroxyapatite molecules. This causes the hydroxyapatite molecule’s molar mass to increase and its solubility to decrease; its chances of being deposited in tumor tissue also increase. This implies that an increased intake of heavy metal salts in organisms can lead to pathological mineralization of breast cancer tissue. Conclusions. Excessive intake of heavy metal salts into the body leads to their involvement in the pathological mineralization of breast cancer tissue. This happens due to these salts bonding to hydroxyapatite molecules, direct sedimentation of proteins and increasing degenerative-necrotic changes in breast cancer tissue as the mineralization process progresses.Item Pathogenetic mechanisms of heavy metals effect on proapoptotic and proliferative potential of breast cancer(Akadémiai Kiadó, Budapest, 2015) Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Кузенко, Євген Вікторович; Кузенко, Евгений Викторович; Kuzenko, Yevhen Viktorovych; Гладченко, Оксана Робертівна; Гладченко, Оксана Робертовна; Gladchenko, Oksana Robertivna; Линдіна, Юлія Миколаївна; Лындина, Юлия Николаевна; Lyndina, Yuliia Mykolaivnaaterials and Methods:Chemical composition was studied with the help of the scanning electron microscope with energy-dispersion spectrometer. Immunohistochemical reaction showed the p53 and Ki-67 receptors expression. The study of DNA fragmentation was performed in agarose gel. Results:There was an interrelation between the accumulations of the trace elements with the degree of cancer malignancy. There were 85% of cases with positive reaction to Ki-67 and 40% cases with positive reaction to p53. We found a moderate correlation between the accumulation of microelements in the breast cancer tissue and the level of proliferative activity. We noted the combination of the increase of DNA fragmentation with the expression of p53 and Ki-67 receptors. Conclusions:The trace elements can cause the initiation and the progression of the tumorous growth, which is expressed in the increased proliferation of tumor cells. This leads to the destabilization of the genetic material which can be expressed in the synthesis of mutant p53 protein. Finally, it leads to the block of apoptosis and regulatory effects of cells. This can cause the tumor progression and the destabilization of the genome, which is reflected in the increased DNA fragmentation.