Наукові видання (НН МІ)
Permanent URI for this collectionhttps://devessuir.sumdu.edu.ua/handle/123456789/132
Browse
12 results
Search Results
Item Decision-making support system for diagnosis of oncopathologies by histological images(Elsevier, 2023) Довбиш, Анатолій Степанович; Довбыш, Анатолий Степанович; Dovbysh, Anatolii Stepanovych; Шелехов, Ігор Володимирович; Шелехов, Игорь Владимирович; Shelekhov, Ihor Volodymyrovych; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Савченко, Тарас Русланович; Савченко, Тарас Русланович; Savchenko, Taras RuslanovychThe aim of the study is to increase the functional efficiency of machine learning decision support system (DSS) for the diagnosis of oncopathology on the basis of tissue morphology. The method of hierarchical information-extreme machine learning of diagnostic DSS is offered. The method is developed within the framework of the functional approach to modeling of natural intelligence cognitive processes at formation and acceptance of classification decisions. This approach, in contrast to neuronal structures, allows diagnostic DSS to adapt to arbitrary conditions of histological imaging and flexibility in retraining the system by expanding the recognition classes alphabet that characterize different structures of tissue morphology. In addition, the decisive rules built within the geometric approach are practically invariant to the multidimensionality of the diagnostic features space. The developed method allows to create information, algorithmic, and software of the automated workplace of the histologist for diagnosing oncopathologies of different genesis. The machine learning method is implemented on the example of diagnosing breast cancerItem Разработка алгоритма поиска опухолевых областей на основе обработки полнослайдовых гистологических изображений рака молочной железы(Белорусский государственный университетинформатики и радиоэлектроники, 2020) Рябцева, С.Н.; Ковалев, В.А.; Малышев, В.Д.; Семеник, И.А.; Деревянко, М.А.; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Довбиш, Анатолій Степанович; Довбыш, Анатолий Степанович; Dovbysh, Anatolii Stepanovych; Савченко, Т.Р.; Романюк, Анатолий Николаевич; Романюк, Анатолій Миколайович; Romaniuk, Anatolii MykolaiovychАнализ полнослайдовых изображений рака молочной железы является крайне трудоемким процессом. Гистологические полнослайдовые изображения обладают рядом особенностей, затрудняющих их разработку: высокая степень разнообразия тканей как на одном изображении, так и между различными изображениями, иерархичность, большой объем графической информации и различные артефакты. В ходе научной работы проведена обработка полнослайдовых изображений ткани рака молочной железы, что включало нормализацию распределения цвета на полнослайдовых гистологических изображениях и выделение области изображения, на которой располагается изучаемый образец ткани, чтобы уменьшить время работы остальных алгоритмов и не анализировать области полнослайдового изображения с фоном. Также разработан и реализован алгоритм поиска похожих для полуавтоматического выделения опухолевых участков с помощью различных дескрипторов изображений.Item The existence of calcifications in breast cancer tissue doesn’t affect expression of HER2-neu protein(Springer, 2020) Коломієць, Олена Олегівна; Коломиец, Елена Олеговна; Kolomiiets, Olena Olehivna; Язиков, Олександр Валерійович; Языков, Александр Валерьевич; Yazykov, Oleksandr Valeriiovych; Лукавенко, Іван Михайлович; Лукавенко, Иван Михайлович; Lukavenko, Ivan Mykhailovych; Андрющенко, Володимир Вікторович; Андрющенко, Владимир Викторович; Andriushchenko, Volodymyr Viktorovych; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman AndriiovychThere is evidence that calcifications in breast cancer (BC) may have an active role in mitogenesis and upregulation of gene expression. To investigate the effect of presence of microcalcifications in breast cancer tissue on Her2-neu expression.Item PIK3CA-mutant circulating tumor DNA in patients with breast cancer(MD Publishing House, 2020) Винниченко, Ігор Олександрович; Винниченко, Игорь Александрович; Vynnychenko, Ihor Oleksandrovych; Привалова, Анастасія Олександрівна; Привалова, Анастасия Александровна; Pryvalova, Anastasiia Oleksandrivna; Винниченко, Олександр Ігорович; Винниченко, Александр Игорович; Vynnychenko, Oleksandr Ihorovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii MykolaiovychDuring the research using digital PCR method, tissue and plasma samples from patients with locally advanced breast cancer were examined for the presence of somatic mutation of PIK3CA in biopsy material and circulating tumor DNA. The presence of PIK3CA gene mutation was detected in 17.24% (5/29) of patients in both biopsy material and plasma. A research of serial samples of patients' plasma revealed changes in the amount of circulating tumor DNA throughout all stages of treatment, which reflected the dynamics of the tumor process. For PIK3CA-mutant breast cancer, ER-positive and Her2/neu-negative tumor phenotypes are characteristic.Item Prognostic value of CEACAM1 in breast cancer in situ(Springer, 2019) Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Singer, B.B.Background & Objectives: Ductal breast cancer can be divided into invasive and non-invasive types (cancer in situ – DCIS). Although there is information about molecular features of these tumours, DCIS remains none fully described pathology. Thus we need to find novel reliable diagnostic markers. One could be the сarcinoembryonic antigen related cell adhesion molecules 1 (CEACAM1). This molecule was described in diverse invasive types of cancers. The aim our study presented here was to characterise the exact expression pattern of CEACAM1 in various types of DCIS.Item Expressed cartilaginous and osseous metaplasia in breast cancer: histological and immunohistochemical aspects(Springer, 2019) Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Гирявенко, Наталія Іванівна; Гирявенко, Наталья Ивановна; Hyriavenko, Nataliia Ivanivna; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Піддубний, Артем Михайлович; Поддубный, Артем Михайлович; Piddubnyi, Artem Mykhailovych; Карпенко, Людмила Іванівна; Карпенко, Людмила Ивановна; Karpenko, Liudmyla Ivanivna; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii MykolaiovychBackground & Objectives: The course of the breast cancer (BC) depends on the differentiation of the tumour, immunophenotype of the neoplastic cells and the qualitative and quantitative characteristics of the tumour microenvironment. Moreover, the expressed secondary changes significantly influence on the progression of the malignant process in the mammory gland. We present the case of the invasive breast cancer (non-specific type) of the right breast in a 64-year-old woman with the features of the expressed rare type of metaplasia.Item Histological and immunohistochemical features of medullary breast cancer(Uniwersytet Jagielloński – Collegium Medicum, 2015) Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Сікора, Владислав Володимирович; Сикора, Владислав Владимирович; Sikora, Vladyslav Volodymyrovych; Линдіна, Юлія Миколаївна; Лындина, Юлия Николаевна; Lyndina, Yuliia Mykolaivna; Panasovska, K.O.Disputes take place among many scientists about the position of MC in the classification of breast cancer. Some say that this kind of tumor is a sign of invasive ductal carcinoma (IDC). Instead, most of modern researchers distinguish MC of the breast as a separate nosological unit. Primarily there were selected 20 cases of MC and 10 cases of IDC (as control group). The immunohistochemical study revealed the presence of ER, PR, HER2/neo, p53, Ki-67, MMP1 and E-cadherin receptors. In the study of receptor status of tumors it was observed that 100% of MC cases were estrogen-, progesterone- and HER2/neu negative. The status of tumors on receptor p53 and Ki-67 was as follows: p53+ status had 80% and Ki-67+ had 85% of tissues of MC. In 75% of cases MC cells expressed marker of adhesion and in 100% of cases cells were receptor-negative for expression of MMP1. The data of the study show that the invasive ductal carcinoma and medullary carcinoma are completely independent and different types of malignancy in the breast. The favorable behavior of medullary cancer is associated with expression of E-cadherin receptors, which are essentially adhesion factor and absence of MMP1 which are factors of metastatic potential of the tumor.Item The role of heavy metal salts in pathological biomineralization of breast cancer tissue(Wroclaw Medical University, 2016) Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Гортинська, Олена Миколаївна; Гортинская, Елена Николаевна; Hortynska, Olena Mykolaivna; Линдіна, Юлія Миколаївна; Лындина, Юлия Николаевна; Lyndina, Yuliia MykolaivnaBackground. The process of pathological biomineralization plays an important part in the morphogenesis of tumors. The role of heavy metal salts in the pathological mineralization of breast cancer tissue should not be ruled out, considering their ability to enter into covalent bonds with calcium salt molecules. Objectives. The aim of the study was to investigate the microelement composition of breast cancer calcifications and the participation of heavy metals in their formation process. Material and Methods. The material for the study consisted of 20 specimens of breast cancer tissue in which calcifications had been found in histological tests (hematoxylin-eozin and alizarin red S staining). The chemical composition of the calcifications was studied using a scanning electron microscope with an energy-dispersive spectrometer. Results. Alizarin red S staining detected the presence of concrements in tumor tissue and rings of calcification around these deposits. Examining the biomineralization with energy dispersive spectrometry showed that along with calcium and phosphorus, it contained microelements such as iron, zinc, copper, chromium and nickel, which can replace calcium ions in the exterior part of hydroxyapatite molecules. This causes the hydroxyapatite molecule’s molar mass to increase and its solubility to decrease; its chances of being deposited in tumor tissue also increase. This implies that an increased intake of heavy metal salts in organisms can lead to pathological mineralization of breast cancer tissue. Conclusions. Excessive intake of heavy metal salts into the body leads to their involvement in the pathological mineralization of breast cancer tissue. This happens due to these salts bonding to hydroxyapatite molecules, direct sedimentation of proteins and increasing degenerative-necrotic changes in breast cancer tissue as the mineralization process progresses.Item The effect of heavy metals accumulation on morphological and immunohistochemical features of breast cancer(Springer, 2016) Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman AndriiovychObjective: The chemical composition determination of neoplastic breast :issue, study of prognostic-important receptors expression in the breast cancer cells, establishing linkages between all the derived indicators.Item Pathogenetic mechanisms of heavy metals effect on proapoptotic and proliferative potential of breast cancer(Akadémiai Kiadó, Budapest, 2015) Романюк, Анатолій Миколайович; Романюк, Анатолий Николаевич; Romaniuk, Anatolii Mykolaiovych; Линдін, Микола Сергійович; Лындин, Николай Сергеевич; Lyndin, Mykola Serhiiovych; Москаленко, Роман Андрійович; Москаленко, Роман Андреевич; Moskalenko, Roman Andriiovych; Кузенко, Євген Вікторович; Кузенко, Евгений Викторович; Kuzenko, Yevhen Viktorovych; Гладченко, Оксана Робертівна; Гладченко, Оксана Робертовна; Gladchenko, Oksana Robertivna; Линдіна, Юлія Миколаївна; Лындина, Юлия Николаевна; Lyndina, Yuliia Mykolaivnaaterials and Methods:Chemical composition was studied with the help of the scanning electron microscope with energy-dispersion spectrometer. Immunohistochemical reaction showed the p53 and Ki-67 receptors expression. The study of DNA fragmentation was performed in agarose gel. Results:There was an interrelation between the accumulations of the trace elements with the degree of cancer malignancy. There were 85% of cases with positive reaction to Ki-67 and 40% cases with positive reaction to p53. We found a moderate correlation between the accumulation of microelements in the breast cancer tissue and the level of proliferative activity. We noted the combination of the increase of DNA fragmentation with the expression of p53 and Ki-67 receptors. Conclusions:The trace elements can cause the initiation and the progression of the tumorous growth, which is expressed in the increased proliferation of tumor cells. This leads to the destabilization of the genetic material which can be expressed in the synthesis of mutant p53 protein. Finally, it leads to the block of apoptosis and regulatory effects of cells. This can cause the tumor progression and the destabilization of the genome, which is reflected in the increased DNA fragmentation.