Видання зареєстровані авторами шляхом самоархівування
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Item Long non-coding RNA MALAT1 gene polymorphism is associated with disease-free survival in bladder cancer patients(Івано-Франківський національний медичний університет, 2020) Волкогон, Андрій Дмитрович; Volkohon, Andrii Dmytrovych; Колногуз, Альона Владиславівна; Kolnohuz, Alona Vladyslavivna; Гарбузова, Вікторія Юріївна; Harbuzova, Viktoriia Yuriivna; Атаман, Олександр Васильович; Ataman, Oleksandr VasylovychThe objective of the research was to study the possible association between MALAT1 gene rs3200401 polymorphism and the survival of patients with bladder cancer and clinicopathological characteristics in bladder cancer. Materials and Methods. The venous blood of 141 patients with transitional cell carcinoma of the urinary bladder was used for study. Genotyping of MALAT1 gene rs3200401 polymorphism was performed by real-time polymerase chain reaction using the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, USA) and Taq-Man Assays (TaqMan©SNP Assay C 3246069 10). Statistical analysis was performed using the SPSS software package (version 17.0). The Kaplan-Meier estimator and Cox regression were used to check the possible association between MALAT1 rs3200401-genotypes and the age of transitional cell carcinoma of the urinary bladder onset. P values < 0.05 were considered as statistically significant. Results. The obtained results revealed that hemoglobin concentration was lower in patients with transitional cell carcinoma of the urinary bladder and rs3200401TT-genotype than in patients with rs3200401CC-genotype (p = 0.024). Herewith, fasting glucose, creatinine concentration, and tumor width were significantly higher in patients with transitional cell carcinoma of the urinary bladder and rs3200401TT-genotype as compared to rs3200401CC-genotype carriers (p = 0.036, p = 0.039, p = 0.028, respectively). The results of survival analysis demonstrated that transitional cell carcinoma of the urinary bladder occurred much later in persons with rs3200401TT-genotype as compared to rs3200401C-allele carriers (log rank p = 0.016), and the risk of transitional cell carcinoma of the urinary bladder onset was lower in individuals with rs3200401TT than in major rs3200401C C-allele carriers (hazard ratio = 0.413; p = 0.047). Conclusions. Rs3200401 polymorphism of MALAT1 gene is associated with disease-free survival in Ukrainian patients with transitional cell carcinoma of the urinary bladder. Transitional cell carcinoma of the urinary bladder occurs later in persons with rs3200401TT-genotype than in individuals with rs3200401CC- and rs3200401CTgenotypes.Item Frequency of allelic variants Lys198Asn polymorhism of endothelin- 1 (EDN-1) gene in the Ukrainian population(Pavlo Josef Šafarik University (Košice, Slovakia), 2016) Олешко, Тетяна Богданівна; Oleshko, Tetiana BohdanivnaStudy of frequency of allelic variants Lys198Asn of EDN−1 gene polymorphism in the Ukrainian population and comparison of the obtained data with the results of research in other populations.Item The associason of vitamin d receptor gene (VDR) polymorphisms with high blood pressure in stroke patients of Ukrainian population(Aluna Publishing, 2020) Obukhova, Olha Anatoliivna; Обухова, Ольга Анатоліївна; Ataman, Oleksandr Vasylovych; Атаман, Олександр Васильович; Zavadska, Maryna Mykolaivna; Завадська, Марина Миколаївна; Piven, Svitlana Mykolaivna; Півень, Світлана Миколаївна; Levchenko, Zoia Mykhailivna; Левченко, Зоя МихайлівнаVenous blood of 170 patients with atherothrombotic ischemic stroke (AIS) and 124 healthy individuals (control group) was used for genotyping. Four polymorphisms (FokI, BsmI, ApaI, TaqI) of gene VDR were examined with PCR-RFLP methodology. Statistical analysis was performed by using SPSS-17.0 program. The correlation of genotypes of polymorphic variants of FokI, BsmI, ApaI and TaqI of the VDR gene with the development of ischemic atherothrombotic stroke in individuals with normal and high blood pressure was detected. Statistical analysis of the obtained data revealed that among carriers of genotypes F/F, b/b, a/a, a/A, and T/T patients with AI have statistically significantly higher incidence of hypertension than patients in the control group. It was found that persons with genotypes F/F, b/b, a/a, a/A, and T/T showed a statistically significant relationship between hypertension and the development of IAS. The application of logistic regression has made it possible to establish that the risk of IAS in people with normal blood pressure and genotype F/f is 3.2 times higher than in normotensive homozygotes for the F-allele.Item Аnalysis of the blood hypercoagulation risk in patients with ischemic atherothrombotic stroke depending of the vdr gene polymorphisms(Aluna publishing, 2023) Обухова, Ольга Анатоліївна; Obukhova, Olha Anatoliivna; Гарбузова, Вікторія Юріївна; Harbuzova, Viktoriia Yuriivna; Завадська, Марина Миколаївна; Zavadska, Maryna Mykolaivna; Левченко, Зоя Михайлівна; Levchenko, Zoia Mykhailivna; Бєсєдіна, Антоніна Анатоліївна; Biesiedina, Antonina Anatoliivna; Гарбузова, Єлізавета Антонівна; Harbuzova, Yelizaveta Antonivna; Сміянова, Юлія Олегівна; Smiianova, Yuliia Olehivna; Сміянов, Владислав Анатолійович; Smiianov, Vladyslav AnatoliiovychМета: дослідження ризику гіперкоагуляції крові у хворих на ішемічний атеротромботичний інсульт залежно від поліморфізму гена VDR. Матеріали і методи. Для генотипування використано кров 170 хворих на ішемічний атеротромботичний інсульт (ІАТІ) та 124 здорових осіб (група контролю). Чотири поліморфізми (FokI, BsmI, ApaI, TaqI) гена VDR досліджували методом ПЛР-ПДРФ. Статистичний аналіз проводили за допомогою програми SPSS-17.0. Результати. Серед хворих на ІАТС, які є носіями генотипу f/f, зареєстровано FokI поліморфізм гена VDR за високим тромбіновим часом та зниженням швидкості спонтанного фібринолізу. В осіб з генотипом B/B, гомозиготним за поліморфним варіантом, BsmI мав значно нижчі середні значення протромбінового та тромбінового часу та збільшував швидкість спонтанного фібринолізу. Було виявлено, що гомозиготи за поліморфізмом А-алелі ApaI мають у 2,7 рази вищий ризик розвитку гіперкоагуляції крові, ніж гомозиготи за а-алелем. Висновки. Біохімічні ознаки синдрому гіперкоагуляції у хворих на ІАТС, які є носіями генотипу f/f поліморфного варіанту FokI та серед гомозигот В/В поліморфного варіанту BsmI та гомозигот за А-алелем поліморфізму AрaI VDR. були зареєстровані гени.Item Revealing the molecular-genetic and clinical predictors of glucocorticoid resistance in patients with hand eczema(Aluna Publishing, 2022) Methkal, A.; Куц, Лариса Вікторівна; Куц, Лариса Викторовна; Kuts, Larysa ViktorivnaTo reveal the possible predictors of the glucocorticoid resistance in patients with hand eczema (HE) based on the demographic, clinical, and molecular-genetic data.Item The association analysis between HOTAIR rs920778 single nucleotide polymorphism and ischemic stroke development in Ukrainian population(Center for Molecular Medicine Cologne (CMMC) - University of Cologne, 2019) Моісеєнко, Каріна Ашотівна; Моисеенко, Карина Ашотовна; Moiseienko, Karina Ashotivna; Harbuzova, Yelizaveta Antonivna; Атаман, Олександр Васильович; Атаман, Александр Васильевич; Ataman, Oleksandr Vasylovych; Гарбузова, Єлізавета Антонівна; Гарбузова, Елизавета АнтоновнаItem Influence of Lys198Asn polymorphism of endothelin-1 gene on ischemic atherothrombotic stroke characteristics(Aluna Publishing, 2020) Олешко, Тетяна Богданівна; Олешко, Татьяна Богдановна; Oleshko, Tetiana Bohdanivna; Чайка, Ірина Сергіївна; Чайка, Ирина Сергеевна; Chaika, Iryna Serhiivna; Олешко, Тетяна Миколаївна; Олешко, Татьяна Николаевна; Oleshko, Tetiana Mykolaivna; Гарбузова, Вікторія Юріївна; Гарбузова, Виктория Юрьевна; Harbuzova, Viktoriia YuriivnaIschemic stroke is one of the most common forms of cerebrovascular disorders and occupies a leading position in the morbidity and mortality of the population worldwide, and is therefore an important medical and social problem. It has been proved that endothelial dysfunction plays a key role in the mechanisms of ischemic stroke development, so this issue has recently become of great importance, has been actively researched by scientists all over the world, and its relevance has not subsided over the yearsItem The association of APAI-Polymorphism of vitamin D receptor gene (VDR) with development of generalized parodontitis in Ukrainian population(ALUNA, 2019) Фоменко, Ілля Геннадійович; Фоменко, Илья Геннадьевич; Fomenko, Illia Hennadiiovych; Гарбузова, Вікторія Юріївна; Гарбузова, Виктория Юрьевна; Harbuzova, Viktoriia Yuriivna; Обухова, Ольга Анатоліївна; Обухова, Ольга Анатольевна; Obukhova, Olha Anatoliivna; Pokhmura, V.V.; Плахтієнко, Інна Олександрівна; Плахтиенко, Инна Александровна; Plakhtiienko, Inna Oleksandrivna; Півень, Світлана Миколаївна; Пивень, Светлана Николаевна; Piven, Svitlana MykolaivnaIntroduction: At present, it is believed that the genetic component is important in the pathogenesis of periodontitis. One of the candidate genes that are of major importance in the development of the disease is the vitamin D receptor gene (VDR). The association of its genetic polymorphisms, in particular Apal, with periodontitis in different populations of the world is proved. The aim: To study the association of the Apal-polymorphism VDR gene with the development of generalized periodontitis in the Ukrainian population. Materials and methods: Patient genotypes were determined by polymerase chain reaction with subsequent analysis of restriction fragment length (PCR-RFLP) from buccal epithelium 116 patients with generalized periodontitis (GP) and 67 individuals of control group. Statistical analysis was performed by using SPSS-17,0 program Results: As a result of the performed studies, it was shown that in the group of patients with GP, the ratio of homozygous for the main allele (a/a), heterozygote (a/A) and homozygote for the minor allele (A/A) was 26 (22,4%), 62 (53,4%), 28 (24,2%), and in control group – 25 (37,3%), 27 (40,3%), 15 (22,4%),respectively. The distribution of genotypes in the comparison groups was not statistically significant (P = 0,084). By the method of binary logistic regression in the framework of the additive inheritance model (a/A vs a/a), a reliable relationship of the genotype with the Apal-polymorphism of the VDR gene was established with the development of generalized periodontitis (Р=0,029). It was shown that in heterozygotes(a/A)the risk of GP in2,208 (95%CI = 1,084-4,496) times is hig her than in homozygotes of the main allele (a/a). After adjusting for age, sex, smoking habit, BMI, the reliability of these results was maintained (P = 0,030)Item Association of Arg389Gly polymorphism of ADRβ1 gene and T393C of GNAS1 gene with risk of development of acute coronary syndrome in patients with arterial hypertension(Lithuanian University of Health Sciences, 2020) Дудченко, Ірина Олександрівна; Дудченко, Ирина Александровна; Dudchenko, Iryna Oleksandrivna; Приступа, Людмила Никодимівна; Приступа, Людмила Никодимовна; Prystupa, Liudmyla Nykodymivna; Фадєєва, Ганна Анатоліївна; Фадеева, Анна Анатольевна; Fadieieva, Hanna Anatoliivna; Moіseienko, Iryna Olehivna; Бондаркова, Анна Миколаївна; Бондаркова, Анна Николаевна; Bondarkova, Anna Mykolaivna; Кучма, Наталія Григорівна; Кучма, Наталья Григорьевна; Kuchma, Nataliia Hryhorivna; Орловський, Олександр Вікторович; Орловский, Александр Викторович; Orlovskyi, Oleksandr Viktorovych; Моісеєнко, Ірина Олегівна; Моисеенко, Ирина ОлеговнаIn our study, we discovered the relationship of the Arg389Gly polymorphism of the ADRβ1 gene and the T393C polymorphism of the GNAS1 gene with the risk of ACS development in the Ukrainian population. Carriers of the Arg389 allele of the polymorphism of the ADRβ1 gene and the C393 allele of the polymorphism of the GNAS1 gene showed a higher risk of ACS development relative to the opposite alleles in these gene polymorphisms.Item G-1639A but Not C1173T VKORC1 Gene Polymorphism Is Related to Ischemic Stroke and Its Various Risk Factors in Ukrainian Population(Hindawi Publishing Corporation, 2016) Дубовик, Євген Іванович; Дубовик, Евгений Иванович; Dubovyk, Yevhen Ivanovych; Гарбузова, Вікторія Юріївна; Гарбузова, Виктория Юрьевна; Harbuzova, Viktoriia Yuriivna; Атаман, Олександр Васильович; Атаман, Александр Васильевич; Ataman, Oleksandr VasylovychVitamin K epoxide reductase complex subunit 1 (VKORC1) is integral 163-amino acid long transmembrane protein which mediates recycling of vitamin K 2,3-epoxide to vitamin K hydroquinone and it is necessary for activation of vitamin K-dependent proteins (VKDPs). Herein, the association between G-1639A (rs9923231) and C1173T (rs9934438) single-nucleotide polymorphisms (SNPs) of the VKORC1 gene and ischemic stroke (IS) was tested in Ukrainian population. Genotyping was performed in 170 IS patients and 124 control subjects (total 294 DNA samples) using PCR-RFLP (polymerase chain reaction with following restriction fragment length polymorphism analysis) method. Our data showed that G-1639A but not C1173T polymorphism was related to IS, regardless of adjustment for age, sex, body mass index, smoking status, and arterial hypertension. The risk for IS in -1639A allele carriers (OR = 2.138, ) was higher than in individuals with G/G genotype. Haplotype analysis demonstrated that -1639G/1173T and -1639A/1173C were related to increased risk for IS (OR = 3.813, and OR = 2.189, 𝑃 = 0.011, resp.), while -1639G/1173C was a protective factor for IS (OR = 0.548, ). Obtained results suggested that -1639A allele can be a possible genetic risk factor for IS in Ukrainian population.