Навчально-науковий медичний інститут (НН МІ)
Permanent URI for this communityhttps://devessuir.sumdu.edu.ua/handle/123456789/40
Browse
8 results
Search Results
Item Effects of the sources of calcium and phosphorus on the structural and functional properties of ceramic coatings on titanium dental implants produced by plasma electrolytic oxidation(Elsevier, 2021) Кириленко, Сергій Дмитрович; Kyrylenko, Serhii Dmytrovych; Warchoł, F.; Олешко, Олександр Миколайович; Oleshko, Oleksandr Mykolaiovych; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Kazek-Kęsik, A.; Корнiєнко, Вiкторiя Володимирiвна; Korniienko, Viktoriia Volodymyrivna; Дейнека, Володимир Миколайович; Deineka, Volodymyr Mykolaiovych; Sowa, M.; Maciej, A.; Michalska, J.; Jakóbik-Kolon, A.; Matuła, I.; Basiaga, M.; Голубнича, Вікторія Миколаївна; Holubnycha, Viktoriia Mykolaivna; Stolarczyk, A.; Pisarek, M.; Mishchenko, O.; Погорєлов, Максим Володимирович; Pohorielov, Maksym Volodymyrovych; Simka, W.Plasma Electrolytic Oxidation (PEO) is as a promising technique to modify metal surfaces by application of oxide ceramic coatings with appropriate physical, chemical and biological characteristics. Therefore, objective of this research was to find the simplest settings, yet able to produce relevant bioactive implant surfaces layers on Ti implants by means of PEO. We show that an electrolyte containing potassium dihydrogen phosphate as a source of P and either calcium hydroxide or calcium formate as a source of Ca in combination with a chelating agent, ethylenediamine tetraacetic acid (EDTA), is suitable for PEO to deliver coatings with desired properties. We determined surface morphology, roughness, wettability, chemical and phase composition of titanium after the PEO process. To investigate biocompatibility and bacterial properties of the PEO oxide coatings we used microbial and cell culture tests. The electrolyte based on Ca(OH)2 and EDTA promotes active crystallization of apatites after PEO processing of the Ti implants. The PEO layers can increase electrochemical corrosion resistance. The PEO can be potentially used for development of bioactive surfaces with increased support of eukaryotic cells while inhibiting attachment and growth of bacteria without use of antibacterial agents.Item Hemostatic performance and biocompatibility of chitosan-based agents in experimental parenchymal bleeding(Elsevier, 2021) Дейнека, Володимир Миколайович; Deineka, Volodymyr Mykolaiovych; Sulaieva, O.; Пернаков, Микола Станіславович; Pernakov, Mykola Stanislavovych; Radwan-Pragłowska, J.; Janus, L.; Корнiєнко, Вiкторiя Володимирiвна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Яновська, Ганна Олександрівна; Yanovska, Hanna Oleksandrivna; Любчак, Ірина Володимирівна; Liubchak, Iryna Volodymyrivna; Юсупова, Азіза Фарходівна; Yusupova, Aziza Farkhodivna; Piątkowski, M.; Zlatska, A.; Погорєлов, Максим Володимирович; Pohorielov, Maksym VolodymyrovychThe uncontrolled parenchymatic bleeding is still a cause of serious complications in surgery and require new effective hemostatic materials. In recent years, numerous chitosan-based materials have been intensively studied for parenchymatic bleeding control but still require to increased safety and effectiveness. The current research is devoted to new hemostatic materials made of natural polymer (chitosan) developed using electrospinning and microwave-assisted methods. Hemostatic performance, biocompatibility, degradation, and in-vivo effectiveness were studied to assess functional properties of new materials. Chitosan-based agents demonstrated considerable hemostatic performance, moderate biodegradation pace and high biocompatibility in vitro. Using the electrospinning-made chitosan-copolymer significantly improved in vivo biocompatibility and degradation of Chitosan-based agents that provides opportunities for its implementation for visceral bleeding management. Chitosan aerogel could be effectively applied in hemostatic patch development due to high antibacterial activity but it is not recommended for visceral application due to moderate inflammatory effect and slow degradation.Item Biological behaviour of chitosan electrospun nanofibrous membranes after different neutralisation methods(Polish Chitin Society, 2022) Корнієнко, Вікторія Володимирівна; Korniienko, Viktoriia Volodymyrivna; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Яновська, Ганна Олександрівна; Yanovska, Hanna Oleksandrivna; Altundal, S.; Дєдкова, Катерина Андріївна; Diedkova, Kateryna Andriivna; Самохін, Євген Олександрович; Samokhin, Yevhen Oleksandrovych; Варава, Юлія Валентинівна; Varava, Yuliia Valentynivna; Голубнича, Вікторія Миколаївна; Holubnycha, Viktoriia Mykolaivna; Viter, R.; Погорєлов, Максим Володимирович; Pohorielov, Maksym VolodymyrovychChitosan electrospun nanofibres were synthesised in two different trifluoroacetic acid (TFA)/dichloromethane (DCM) solvent ratios and then neutralised in aqueous and ethanol sodium-based solutions (NaOH and Na2CO3) to produce insoluble materials with enhanced biological properties for regenerative and tissue engineering applications. Structural, electronic, and optical properties and the swelling capacity of the prepared nanofibre membrane were studied by scanning electron microscopy, Fourier-transform infrared spectroscopy, and photoluminescence. Cell viability (with the U2OS cell line) and antibacterial properties (against Staphylococcus aureus and Escherichia coli) assays were used to assess the biomedical potential of the neutralised chitosan nanofibrous membranes. A 7:3 TFA/DCM ratio allows for an elaborate nanofibrous membrane with a more uniform fibre size distribution. Neutralisation in aqueous NaOH only maintains a partial fibrous structure. At the same time, neutralisation in NaOH ethanol-water maintains the structure during 1 month of degradation in phosphate-buffered saline and distilled water. All membranes demonstrate high biocompatibility, but neutralisation in ethanol solutions affects cell proliferation on materials made with 9:1 TFA/DCM. The prepared nanofibrous mats could constrain the growth of both gram-positive and gram-negative microorganisms, but 7:3 TFA/DCM membranes inhibited bacterial growth more efficiently. Based on structural, degradation, and biological properties, 7:3 TFA/DCM chitosan nanofibrous membranes neutralised by 70% ethanol/30% aqueous NaOH exhibit potential for biomedical and tissue engineering applications.Item Fabrication and Characterization of Electrospun Chitosan/Polylactic Acid (CH/PLA) Nanofiber Scaffolds for Biomedical Application(MDPI, 2023) Самохін, Євген Олександрович; Samokhin, Yevhen Oleksandrovych; Варава, Юлія Валентинівна; Varava, Yuliia Valentynivna; Дєдкова, Катерина Андріївна; Diedkova, Kateryna Andriivna; Yanko, I.; Гусак, Євгенія Володимирівна; Husak, Yevheniia Volodymyrivna; Radwan-Pragłowska, J.; Погорєлова, Оксана Сергіївна; Pohorielova, Oksana Serhiivna; Janus, Ł.; Погорєлов, Максим Володимирович; Pohorielov, Maksym Volodymyrovych; Корнiєнко, Вiкторiя Володимирiвна; Korniienko, Viktoriia VolodymyrivnaThe present study demonstrates a strategy for preparing porous composite fibrous materials with superior biocompatibility and antibacterial performance. The findings reveal that the incorporation of PEG into the spinning solutions significantly influences the fiber diameters, morphology, and porous area fraction. The addition of a hydrophilic homopolymer, PEG, into the Ch/PLA spinning solution enhances the hydrophilicity of the resulting materials. The hybrid fibrous materials, comprising Ch modified with PLA and PEG as a co-solvent, along with post-treatment to improve water stability, exhibit a slower rate of degradation (stable, moderate weight loss over 16 weeks) and reduced hydrophobicity (lower contact angle, reaching 21.95 ± 2.17°), rendering them promising for biomedical applications. The antibacterial activity of the membranes is evaluated against Staphylococcus aureus and Escherichia coli, with PEG-containing samples showing a twofold increase in bacterial reduction rate. In vitro cell culture studies demonstrated that PEG-containing materials promote uniform cell attachment, comparable to PEG-free nanofibers. The comprehensive evaluation of these novel materials, which exhibit improved physical, chemical, and biological properties, highlights their potential for biomedical applications in tissue engineering and regenerative medicine.Item Bioactivity Performance of Pure Mg after Plasma Electrolytic Oxidation in Silicate-Based Solutions(MDPI, 2021) Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Michalska, J.; Олешко, Олександр Миколайович; Олешко, Александр Николаевич; Oleshko, Oleksandr Mykolaiovych; Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Grundsteins, K.; Дригваль, Богдан Олександрович; Дрыгваль, Богдан Александрович; Dryhval, Bohdan Oleksandrovych; Altundal, S.; Mishchenko, O.; Вітер, Роман Віталійович; Ветер, Роман Витальевич; Viter, Roman Vitaliiovych; Simka, W.The biodegradable metals, including magnesium (Mg), are a convenient alternative to permanent metals but fast uncontrolled corrosion limited wide clinical application. Formation of a barrier coating on Mg alloys could be a successful strategy for the production of a stable external layer that prevents fast corrosion. Our research was aimed to develop an Mg stable oxide coating using plasma electrolytic oxidation (PEO) in silicate-based solutions. 99.9% pure Mg alloy was anodized in electrolytes contained mixtures of sodium silicate and sodium fluoride, calcium hydroxide and sodium hydroxide. Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), contact angle (CA), Photoluminescence analysis and immersion tests were performed to assess structural and long-term corrosion properties of the new coating. Biocompatibility and antibacterial potential of the new coating were evaluated using U2OS cell culture and the grampositive Staphylococcus aureus (S. aureus, strain B 918). PEO provided the formation of a porous oxide layer with relatively high roughness. It was shown that Ca(OH)2 was a crucial compound for oxidation and surface modification of Mg implants, treated with the PEO method. The addition of Ca2+ ions resulted in more intense oxidation of the Mg surface and growth of the oxide layer with a higher active surface area. Cell culture experiments demonstrated appropriate cell adhesion to all investigated coatings with a significantly better proliferation rate for the samples treated in Ca(OH)2 - containing electrolyte. In contrast, NaOH-based electrolyte provided more relevant antibacterial effects but did not support cell proliferation. In conclusion, it should be noted that PEO of Mg alloy in silicate baths containing Ca(OH)2 provided the formation of stable biocompatible oxide coatings that could be used in the development of commercial degradable implantsItem Formation of a Bacteriostatic Surface on ZrNb Alloy via Anodization in a Solution Containing Cu Nanoparticles(MDPI, 2020) Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Олешко, Олександр Миколайович; Олешко, Александр Николаевич; Oleshko, Oleksandr Mykolaiovych; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Дейнека, Володимир Миколайович; Дейнека, Владимир Николаевич; Deineka, Volodymyr Mykolaiovych; Голубнича, Вікторія Миколаївна; Голубничая, Виктория Николаевна; Holubnycha, Viktoriia Mykolaivna; Mishchenko, O.; Kazek-Kęsik, A.; Jakobik-Kolon, A.; Пшеничний, Роман Миколайович; Пшеничный, Роман Николаевич; Pshenychnyi, Roman Mykolaiovych; Leśniak-Ziółkowska, K.; Kalinkevich, O.; Kalinkevich, A.; Pisarek, M.; Simka, W.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychHigh strength, excellent corrosion resistance, high biocompatibility, osseointegration ability, and low bacteria adhesion are critical properties of metal implants. Additionally, the implant surface plays a critical role as the cell and bacteria host, and the development of a simultaneously antibacterial and biocompatible implant is still a crucial challenge. Copper nanoparticles (CuNPs) could be a promising alternative to silver in antibacterial surface engineering due to low cell toxicity. In our study, we assessed the biocompatibility and antibacterial properties of a PEO (plasma electrolytic oxidation) coating incorporated with CuNPs (Cu nanoparticles). The structural and chemical parameters of the CuNP and PEO coating were studied with TEM/SEM (Transmission Electron Microscopy/Scanning Electron Microscopy), EDX (Energy-Dispersive X-ray Dpectroscopy), and XRD (X-ray Diffraction) methods. Cell toxicity and bacteria adhesion tests were used to prove the surface safety and antibacterial properties. We can conclude that PEO on a ZrNb alloy in Ca–P solution with CuNPs formed a stable ceramic layer incorporated with Cu nanoparticles. The new surface provided better osteoblast adhesion in all time-points compared with the nontreated metal and showed medium grade antibacterial activities. PEO at 450 V provided better antibacterial properties that are recommended for further investigation.Item In Vitro Biological Characterization of Silver-Doped Anodic Oxide Coating on Titanium(MDPI, 2020) Олешко, Олександр Миколайович; Олешко, Александр Николаевич; Oleshko, Oleksandr Mykolaiovych; Любчак, Ірина Володимирівна; Любчак, Ирина Владимировна; Liubchak, Iryna Volodymyrivna; Гусак, Євгенія Володимирівна; Гусак, Евгения Владимировна; Husak, Yevheniia Volodymyrivna; Корнiєнко, Вiкторiя Володимирiвна; Корниенко, Виктория Владимировна; Korniienko, Viktoriia Volodymyrivna; Юсупова, Азіза Фарходівна; Юсупова, Азиза Фархадовна; Yusupova, Aziza Farkhodivna; Олешко, Тетяна Богданівна; Олешко, Татьяна Богдановна; Oleshko, Tetiana Bohdanivna; Banasiuk, R.; Szkodo, M.; Matros-Taranets, I.; Kazek-Kęsik, A.; Simka, W.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychDespite the high biocompatibility and clinical effectiveness of Ti-based implants, surface functionalization (with complex osteointegrative/antibacterial strategies) is still required. To enhance the dental implant surface and to provide additional osteoinductive and antibacterial properties, plasma electrolytic oxidation of a pure Ti was performed using a nitrilotriacetic acid (NTA)-based Ag nanoparticles (AgNP)-loaded calcium–phosphate solution. Chemical and structural properties of the surface-modified titanium were assessed using scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) and contact angle measurement. A bacterial adhesion test and cell culture biocompatibility with collagen production were performed to evaluate biological effectiveness of the Ti after the plasma electrolytic process. The NTA-based calcium–phosphate solution with Ag nanoparticles (AgNPs) can provide formation of a thick, porous plasma electrolytic oxidation (PEO) layer enriched in silver oxide. Voltage elevation leads to increased porosity and a hydrophilic nature of the newly formed ceramic coating. The silver-enriched PEO layer exhibits an effective antibacterial effect with high biocompatibility and increased collagen production that could be an effective complex strategy for dental and orthopedic implant development.Item New Zr-Ti-Nb Alloy for Medical Application: Development, Chemical and Mechanical Properties, and Biocompatibility(MDPI, 2020) Mishchenko, O.; Ovchynnykov, O.; Kapustian, O.; Погорєлов, Максим Володимирович; Погорелов, Максим Владимирович; Pohorielov, Maksym VolodymyrovychThe concept of mechanical biocompatibilities is considered an important factor for orthopedics and dental implants. The high Young modulus of traditional Ti-based alloys can lead to stress-shielding syndrome and late postoperative complications. The development of new Al- and V-free Ti alloys with a low elastic modulus is a critical task for implantology. Despite the relatively low Young modulus and appropriate biological response of metastable beta-Ti alloys, their production requires complex metallurgical solutions and a high final cost that limit commercial application. The current research aimed to develop a Zr-Ti-Nb system with a low Young modulus suitable for biomedical application, including orthopedics and dental implantology. Two different charges were used for new alloy production with melting in a vacuum-arc furnace VDP-1 under atmospheric control (argon + helium) with a non-consumable tungsten electrode and a water-cooled copper crystallizer. Post-treatment included a forging-rolling process to produce a bar suitable for implant production. SEM with EDX and the mechanical parameters of the new alloy were evaluated, and a cell culture experiment provided a biocompatibility assessment. The chemical composition of the new alloy can be represented as 59.57-19.02-21.41 mass% of Zr-Ti-Nb. The mechanical properties are characterized by an extremely low Young modulus—27,27 GPa for the alloy and 34.85 GPa for the bar. The different master alloys used for Zr-Ti-Nb production did not affect the chemical compound and mechanical parameters so it was possible to use affordable raw materials to decrease the final price of the new product. The cell culture experiment demonstrated a full biocompatibility, indicating that this new alloy can be used for dental and orthopedics implant production.