Periodontitis and atherosclerosis – mechanisms of association through matrix metalloproteinase 1 expression
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2014
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Wroclaw Medical University and Polish Dental Society
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Abstract
Wprowadzenie. Od dawna jest postulowany związek między zapaleniem przyzębia a miażdżycą naczyń i jej powikłaniami.
Cel pracy. Analiza ekspresji metaloproteinazy 1 (MMP-1) w nabłonku dziąsłowym i podnabłonkowej tkance łącznej u osób, które zmarły z powodu powikłań miażdżycy naczyń.
Materiał i metody. Zbadano 20 wycinków dziąsła osób, które zmarły z powodu powikłań miażdżycy w szpitalu
w S umie na Ukrainie. Grupę kontrolną stanowiło 9 wycinków dziąsła osób zmarłych z innych przyczyn. Ekspresję MMP-1 oceniano za pomocą badania immunohistochemicznego i badania wykonanego z zastosowaniem mikroskopii fluorescencyjnej.
Wyniki. Immunoekspresja MMP-1 w grupie z miażdżycą była potwierdzona przez brązowe zabarwienie cytoplazmy keratynocytów w nabłonku dziąsłowym i komórkach nacieku leukocytarnego w podnabłonkowej tkance łącznej. Ekspresja MMP-1 w warstwach nabłonka dziąsłowego dotyczyła 95,8 ± 2,43% keratynocytów i była istotnie
Background. The association between periodontitis and atherosclerosis and its complications has long been postulated. Objectives. The objective of this study was to analyze the expression of MMP-1 in gingival epithelium. Material and Methods. 29 pieces of periodontal tissues of patients who had died in Sumy Regional Hospital were evaluated for revealing MMP-1 by immunohistochemistry and fluorescent microscopy. Results. Immunoexpression of MMP-1 (atherosclerotic group) was confirmed by the presence of brown stained cytoplasm epithelial layers and leukocytic infiltration. Immunoexpression of MMP-1 in epithelial layers showed 95.8 ± 2.43% (P < 0.001) positive cells. In the lamina propria leukocytic infiltration, immunoreactivity of MMP-1 was classified with a score of 19 in most atherosclerotic cases, that is 41.21 ± 3.86% (P < 0.05). Immunoexpression of MMP-1 in epithelial layers showed the result of 35.1 ± 4.89% (P < 0.05) positive cells and immunoexpression of MMP-1 in leukocytic infiltration showed 48.23 ± 5.24% (P < 0.05). In general, MMP-1 staining seemed to be more intense in the granular cell layer. Conclusions. MMP-1 plays an important role in the biology of periodontal disease. An increased concentration of MMP-1 in the epithelial layers and leukocytic infiltration of group I patients suggests that the expression of MMP-1 contributes to epithelial invasion of collagen matrix in case of atherosclerosis. This mechanism might contribute to explaining the association between major cardiovascular diseases and oral infections.
Background. The association between periodontitis and atherosclerosis and its complications has long been postulated. Objectives. The objective of this study was to analyze the expression of MMP-1 in gingival epithelium. Material and Methods. 29 pieces of periodontal tissues of patients who had died in Sumy Regional Hospital were evaluated for revealing MMP-1 by immunohistochemistry and fluorescent microscopy. Results. Immunoexpression of MMP-1 (atherosclerotic group) was confirmed by the presence of brown stained cytoplasm epithelial layers and leukocytic infiltration. Immunoexpression of MMP-1 in epithelial layers showed 95.8 ± 2.43% (P < 0.001) positive cells. In the lamina propria leukocytic infiltration, immunoreactivity of MMP-1 was classified with a score of 19 in most atherosclerotic cases, that is 41.21 ± 3.86% (P < 0.05). Immunoexpression of MMP-1 in epithelial layers showed the result of 35.1 ± 4.89% (P < 0.05) positive cells and immunoexpression of MMP-1 in leukocytic infiltration showed 48.23 ± 5.24% (P < 0.05). In general, MMP-1 staining seemed to be more intense in the granular cell layer. Conclusions. MMP-1 plays an important role in the biology of periodontal disease. An increased concentration of MMP-1 in the epithelial layers and leukocytic infiltration of group I patients suggests that the expression of MMP-1 contributes to epithelial invasion of collagen matrix in case of atherosclerosis. This mechanism might contribute to explaining the association between major cardiovascular diseases and oral infections.
Keywords
атеросклероз, atherosclerosis, імуногістохімія, иммуногистохимия, immunohistochemistry, MMP-1, періодонтит, периодонтит, periodontitis
Citation
Kuzenko, E.V. Periodontitis and atherosclerosis – mechanisms of association through matrix metalloproteinase 1 expression / E.V. Kuzenko, A.M. Romaniuk, D. Horobchenko // Dent. Med. Probl. - 2014. - Vol. 51, No.2. - P. 187–192.